DEAH box RNA helicase DHX38 associates with satellite I noncoding RNA involved in chromosome segregation.

Cell Line Centromere / genetics Chromosome Segregation DEAD-box RNA Helicases / metabolism DNA, Satellite Gene Knockdown Techniques Humans RNA Splicing Factors / metabolism RNA, Untranslated / genetics
chromosome segregation noncoding RNA ribonucleoprotein (RNP) complex

Journal

Genes to cells : devoted to molecular & cellular mechanisms
ISSN: 1365-2443
Titre abrégé: Genes Cells
Pays: England
ID NLM: 9607379

Informations de publication

Date de publication:
Aug 2019
Historique:
received: 11 03 2019
revised: 29 05 2019
accepted: 01 06 2019
pubmed: 6 6 2019
medline: 10 1 2020
entrez: 6 6 2019
Statut: ppublish

Résumé

Noncoding (nc) RNA called satellite I is transcribed from the human centromere region. Depletion of this ncRNA results in abnormal nuclear morphology because of defects in chromosome segregation. Some protein factors interact with this ncRNA and function as a component of a nc ribonucleoprotein (RNP) complex in mitotic regulation. Here, we found that DHX38, a pre-mRNA splicing-related DEAH box RNA helicase, interacts with satellite I ncRNA. Depletion of DHX38 resulted in defective chromosome segregation similar to knockdown of satellite I ncRNA. Interaction between DHX38 and ncRNA was interphase-specific, but DHX38 depletion affected the function of Aurora B, which associated with satellite I ncRNA at mitotic phase. Based on these findings, we suggest that DHX38 has a role in mitotic regulation as a component of the satellite I ncRNP complex at interphase.

Identifiants

DOI: 10.1111/gtc.12707 PMID: 31166646
pubmed: 31166646
doi: 10.1111/gtc.12707
doi:

Substances chimiques

DNA, Satellite 0
RNA Splicing Factors 0
RNA, Untranslated 0
DHX38 protein, human EC 3.6.1.-
DEAD-box RNA Helicases EC 3.6.4.13

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

585-590

Subventions

Organisme : The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : 26870442
Organisme : The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : 24770168
Organisme : The Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : 23112716
Organisme : The Japan Agency for Medical Research and Development (CREST)
ID : JP16gm0510007

Informations de copyright

© 2019 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.

Auteurs

Kanako Nishimura (K)

Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.

Yukiko Cho (Y)

Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.

Kazuaki Tokunaga (K)

Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto, Japan.

Mitsuyoshi Nakao (M)

Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto, Japan.

Tokio Tani (T)

Faculty of Advanced Science and Technology, Kumamoto University, Kumamoto, Japan.
ORCID: 0000-0003-4407-5541

Takashi Ideue (T)

Faculty of Advanced Science and Technology, Kumamoto University, Kumamoto, Japan.
ORCID: 0000-0001-7277-1785

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › DEAH box RNA helicase DHX38 associates with...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Centromère › DEAH box RNA helicase DHX38 associates with...
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Ségrégation des chromosomes › DEAH box RNA helicase DHX38 associates with...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › RNA helicases › DEAD-box RNA helicases › DEAH box RNA helicase DHX38 associates with...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › ADN satellite › DEAH box RNA helicase DHX38 associates with...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Techniques de knock-down de gènes › DEAH box RNA helicase DHX38 associates with...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › DEAH box RNA helicase DHX38 associates with...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Facteurs d'épissage des ARN › DEAH box RNA helicase DHX38 associates with...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › DEAH box RNA helicase DHX38 associates with...