Parthenogenesis in a captive Asian water dragon (Physignathus cocincinus) identified with novel microsatellites.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2019
Historique:
received: 24 12 2018
accepted: 12 05 2019
entrez: 6 6 2019
pubmed: 6 6 2019
medline: 26 2 2020
Statut: epublish

Résumé

Reptiles show varying degrees of facultative parthenogenesis. Here we use genetic methods to determine that an isolated, captive female Asian water dragon produced at least nine offspring via parthenogenesis. We identified microsatellites for the species from shotgun genomic sequences, selected and optimized primer sets, and tested all of the offspring for a set of seven microsatellites that were heterozygous in the mother. We verified that the seven loci showed high levels of polymorphism in four wild Asian water dragons from Vietnam. In all cases, the offspring (unhatched, but developed eggs, or hatched young) had only a single allele at each locus, and contained only alleles present in the mother's genotype (i.e., were homozygous or hemizygous). The probability that our findings resulted from the female mating with one or more males is extremely small, indicating that the offspring were derived from a single female gamete (either alone or via duplication and/or fusion) and implicating parthenogenesis. This is the first documented case of parthenogenesis in the Squamate family Agamidae.

Identifiants

pubmed: 31166974
doi: 10.1371/journal.pone.0217489
pii: PONE-D-18-36555
pmc: PMC6550409
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0217489

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Kyle L Miller (KL)

Department of Animal Care Sciences, Smithsonian's National Zoological Park Washington, District of Columbia, United States of America.

Susette Castañeda Rico (S)

Center for Conservation Genomics, Smithsonian Conservation Biology Institute, National Zoological Park, Washington, District of Columbia, United States of America.
George Mason University, Fairfax, Virginia, United States of America.

Carly R Muletz-Wolz (CR)

Center for Conservation Genomics, Smithsonian Conservation Biology Institute, National Zoological Park, Washington, District of Columbia, United States of America.

Michael G Campana (MG)

Center for Conservation Genomics, Smithsonian Conservation Biology Institute, National Zoological Park, Washington, District of Columbia, United States of America.

Nancy McInerney (N)

Center for Conservation Genomics, Smithsonian Conservation Biology Institute, National Zoological Park, Washington, District of Columbia, United States of America.

Lauren Augustine (L)

Department of Animal Care Sciences, Smithsonian's National Zoological Park Washington, District of Columbia, United States of America.
Saint Louis Zoo, One Government Drive, Saint Louis, Missouri, United States of America.

Celine Frere (C)

University of the Sunshine Coast, Sippy Downs, Queensland, Australia.

Alan M Peters (AM)

Department of Animal Care Sciences, Smithsonian's National Zoological Park Washington, District of Columbia, United States of America.

Robert C Fleischer (RC)

Center for Conservation Genomics, Smithsonian Conservation Biology Institute, National Zoological Park, Washington, District of Columbia, United States of America.

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Classifications MeSH