Robo2 regulates synaptic oxytocin content by affecting actin dynamics.


Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
10 06 2019
Historique:
received: 30 01 2019
accepted: 08 06 2019
pubmed: 11 6 2019
medline: 3 3 2020
entrez: 11 6 2019
Statut: epublish

Résumé

The regulation of neuropeptide level at the site of release is essential for proper neurophysiological functions. We focused on a prominent neuropeptide, oxytocin (OXT) in the zebrafish as an in vivo model to visualize and quantify OXT content at the resolution of a single synapse. We found that OXT-loaded synapses were enriched with polymerized actin. Perturbation of actin filaments by either cytochalasin-D or conditional Cofilin expression resulted in decreased synaptic OXT levels. Genetic loss of

Identifiants

pubmed: 31180321
doi: 10.7554/eLife.45650
pii: 45650
pmc: PMC6590984
doi:
pii:

Substances chimiques

Actins 0
Intracellular Signaling Peptides and Proteins 0
Receptors, Immunologic 0
Slit3 protein, zebrafish 0
Zebrafish Proteins 0
robo2 protein, zebrafish 0
Green Fluorescent Proteins 147336-22-9
Oxytocin 50-56-6
cdc42 GTP-Binding Protein EC 3.6.5.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Israel Science Foundation
ID : 1511/16
Pays : International
Organisme : Israel Science Foundation
ID : 2137/16
Pays : International
Organisme : Minerva Foundation
ID : Minerva Stiftung
Pays : International
Organisme : United States-Israel Binational Science Foundation
ID : 2017325
Pays : International
Organisme : Minerva Foundation
ID : (Minerva Stiftung)
Pays : International

Commentaires et corrections

Type : ErratumIn

Informations de copyright

© 2019, Anbalagan et al.

Déclaration de conflit d'intérêts

SA, JB, MG, LG, RR, TD, ES, GL No competing interests declared

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Auteurs

Savani Anbalagan (S)

Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

Janna Blechman (J)

Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

Michael Gliksberg (M)

Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

Ludmila Gordon (L)

Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

Ron Rotkopf (R)

Bioinformatics Unit, LSCF, Weizmann Institute of Science, Rehovot, Israel.
Electron Microscopy Unit, Weizmann Institute of Science, Rehovot, Israel.

Tali Dadosh (T)

Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.

Eyal Shimoni (E)

Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.

Gil Levkowitz (G)

Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

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Classifications MeSH