AhR regulates the expression of human cytochrome P450 1A1 (CYP1A1) by recruiting Sp1.
Basic Helix-Loop-Helix Transcription Factors
/ genetics
Carcinogens
/ toxicity
Cytochrome P-450 CYP1A1
/ genetics
Gene Expression Regulation
/ genetics
Hep G2 Cells
Humans
Intestinal Mucosa
/ metabolism
Kidney
/ metabolism
Liver
/ metabolism
Metabolic Detoxication, Phase I
/ genetics
Neoplasms
/ chemically induced
Promoter Regions, Genetic
Protein Binding
/ genetics
Receptors, Aryl Hydrocarbon
/ genetics
Sp1 Transcription Factor
/ genetics
Transcriptional Activation
/ genetics
CYP1A1
GC box
XRE
basal expression
coordinated regulation
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
19
12
2018
revised:
09
04
2019
accepted:
11
06
2019
pubmed:
15
6
2019
medline:
12
6
2020
entrez:
15
6
2019
Statut:
ppublish
Résumé
Cytochrome P450 1A1 (CYP1A1) is abundant in the kidney, liver, and intestine and is involved in the phase I metabolism of numerous endogenous and exogenous compounds. Therefore, exploring the regulatory mechanism of its basal expression in humans is particularly important to understand the bioactivation of several procarcinogens to their carcinogenic derivatives. Site-specific mutagenesis and deletion of the transcription factor binding site determined the core cis-acting elements in the human CYP1A1 proximal and distal promoter regions. The proximal promoter region [overlapping xenobiotic-responsive element (XRE) and GC box sequences] determined the basal expression of CYP1A1. In human hepatocellular carcinoma cells (HepG2) with aryl hydrocarbon receptor (AhR) or specificity protein 1 (Sp1) knockdown, we confirmed that AhR and Sp1 are involved in basal CYP1A1 expression. In HepG2 cells overexpressing either AhR or Sp1, AhR determined the proximal transactivation of basal CYP1A1 expression. Via DNA affinity precipitation assays and ChIP, we found that AhR bound to the promoter and recruited Sp1 to transactivate CYP1A1 expression. The coordinated interaction between Sp1 and AhR was identified to be DNA mediated. Our work revealed a basal regulatory mechanism of an interesting human gene by which AhR interacts with Sp1 through DNA and recruits Sp1 to regulate basal CYP1A1 expression.
Substances chimiques
AHR protein, human
0
Basic Helix-Loop-Helix Transcription Factors
0
Carcinogens
0
Receptors, Aryl Hydrocarbon
0
Sp1 Transcription Factor
0
SP1 protein, human
0
CYP1A1 protein, human
EC 1.14.14.1
Cytochrome P-450 CYP1A1
EC 1.14.14.1
Banques de données
GENBANK
['NM_000499', 'NM_138473', 'NM_001621']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4215-4231Subventions
Organisme : Department of Education of Guangdong Province
ID : 2017KCXTD001
Pays : International
Organisme : Natural Science Foundation of Guangdong Province
ID : 2015A030312005
Pays : International
Organisme : Science and Technology Program of Guangzhou
ID : 201804020067
Pays : International
Organisme : Science and Technology Program of Guangzhou
ID : 201607010177
Pays : International
Informations de copyright
© 2019 Federation of European Biochemical Societies.
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