Functional characterization of an invertebrate gonadotropin-releasing hormone receptor in the Yesso scallop Mizuhopecten yessoensis.
ACP
AKH
CRZ
GnRH
Mollusc
Receptor assay
Second messenger
Journal
General and comparative endocrinology
ISSN: 1095-6840
Titre abrégé: Gen Comp Endocrinol
Pays: United States
ID NLM: 0370735
Informations de publication
Date de publication:
01 10 2019
01 10 2019
Historique:
received:
09
04
2019
revised:
22
05
2019
accepted:
10
06
2019
pubmed:
15
6
2019
medline:
18
1
2020
entrez:
15
6
2019
Statut:
ppublish
Résumé
The neuropeptide control of bivalve reproduction with particular reference to gonadotropin-releasing hormone (invGnRH) is a frontier yet to be investigated. Bivalves are unique because they have two forms of the invGnRH peptide; however, there has been no functional characterization of the peptide-receptor pair. Therefore, the identification of a cognate receptor is a preliminary step toward exploring the biological roles of invGnRHs in bivalves. In this study, we functionally characterize an invGnRH receptor (invGnRHR) of a bivalve, the Yesso scallop Mizuhopecten yessoensis. In the receptor assay, HEK293 cells were transfected to transiently express the M. yessoensis invGnRHR (my-invGnRHR), which was found to be localized on the plasma membrane, confirming that my-invGnRHR, similar to other G-protein-coupled receptors, functions as a membrane receptor. Using both forms of invGnRH as ligands in a function-receptor assay, my-invGnRH11aa-NH
Identifiants
pubmed: 31199924
pii: S0016-6480(19)30171-6
doi: 10.1016/j.ygcen.2019.06.005
pii:
doi:
Substances chimiques
Peptides
0
Receptors, LHRH
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113201Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.