Structural and biochemical characterization of 20β-hydroxysteroid dehydrogenase from
Bacterial Proteins
/ chemistry
Bifidobacterium adolescentis
/ enzymology
Crystallography, X-Ray
Hydrocortisone
/ chemistry
Hydroxysteroid Dehydrogenases
/ chemistry
Kinetics
Mutagenesis, Site-Directed
NAD
/ chemistry
Protein Binding
Protein Structure, Secondary
Protein Structure, Tertiary
Recombinant Proteins
/ biosynthesis
Substrate Specificity
20β-hydroxysteroid dehydrogenase
Bifidobacteria
C-20 reduction
Steroid-17,20-desmolase
androgen
cortisol
glucocorticoid
microbiome
oxidation–reduction (redox)
probiotic
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
09 08 2019
09 08 2019
Historique:
received:
16
05
2019
revised:
11
06
2019
pubmed:
19
6
2019
medline:
17
3
2020
entrez:
19
6
2019
Statut:
ppublish
Résumé
Anaerobic bacteria inhabiting the human gastrointestinal tract have evolved various enzymes that modify host-derived steroids. The bacterial steroid-17,20-desmolase pathway cleaves the cortisol side chain, forming pro-androgens predicted to impact host physiology. Bacterial 20β-hydroxysteroid dehydrogenase (20β-HSDH) regulates cortisol side-chain cleavage by reducing the C-20 carboxyl group on cortisol, yielding 20β-dihydrocortisol. Recently, the gene encoding 20β-HSDH in
Identifiants
pubmed: 31209107
pii: S0021-9258(20)30155-1
doi: 10.1074/jbc.RA119.009390
pmc: PMC6690682
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Recombinant Proteins
0
NAD
0U46U6E8UK
Hydroxysteroid Dehydrogenases
EC 1.1.-
Hydrocortisone
WI4X0X7BPJ
Banques de données
PDB
['3R1I', '6M9U', '6OW4', '4O0L', '3CTM']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12040-12053Informations de copyright
© 2019 Doden et al.
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