The Role of Transcription Factors and Nuclear Pore Proteins in Controlling the Spatial Organization of the Yeast Genome.
chromatin
chromosome
inter-chromosomal clustering
nuclear architecture
nuclear pore complex
transcription
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
17 06 2019
17 06 2019
Historique:
received:
15
10
2018
revised:
18
04
2019
accepted:
10
05
2019
entrez:
19
6
2019
pubmed:
19
6
2019
medline:
25
1
2020
Statut:
ppublish
Résumé
Loss of nuclear pore complex (NPC) proteins, transcription factors (TFs), histone modification enzymes, Mediator, and factors involved in mRNA export disrupts the physical interaction of chromosomal sites with NPCs. Conditional inactivation and ectopic tethering experiments support a direct role for the TFs Gcn4 and Nup2 in mediating interaction with the NPC but suggest an indirect role for factors involved in mRNA export or transcription. A conserved "positioning domain" within Gcn4 controls interaction with the NPC and inter-chromosomal clustering and promotes transcription of target genes. Such a function may be quite common; a comprehensive screen reveals that tethering of most yeast TFs is sufficient to promote targeting to the NPC. While some TFs require Nup100, others do not, suggesting two distinct targeting mechanisms. These results highlight an important and underappreciated function of TFs in controlling the spatial organization of the yeast genome through interaction with the NPC.
Identifiants
pubmed: 31211995
pii: S1534-5807(19)30421-6
doi: 10.1016/j.devcel.2019.05.023
pmc: PMC6613381
mid: NIHMS1529791
pii:
doi:
Substances chimiques
Basic-Leucine Zipper Transcription Factors
0
Chromatin
0
GCN4 protein, S cerevisiae
0
NUP2 protein, S cerevisiae
0
Nuclear Pore Complex Proteins
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
936-947.e4Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM080484
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118712
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008061
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008382
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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