Unilateral L4-dorsal root ganglion stimulation evokes pain relief in chronic neuropathic postsurgical knee pain and changes of inflammatory markers: part II whole transcriptome profiling.
Aged
Biomarkers
/ blood
Chronic Pain
/ blood
Complex Regional Pain Syndromes
/ blood
Cytokines
/ blood
Female
Ganglia, Spinal
/ physiology
Gene Expression Profiling
Humans
Inflammation
/ blood
Inflammation Mediators
/ blood
Knee
/ pathology
Male
Metabolic Networks and Pathways
/ genetics
Middle Aged
Neuralgia
/ blood
Pain Management
/ methods
Pain, Postoperative
/ blood
Saliva
/ chemistry
Transcutaneous Electric Nerve Stimulation
/ methods
Chronic neuropathic pain
Dorsal root ganglion stimulation
Gene expression
Transcriptome
Journal
Journal of translational medicine
ISSN: 1479-5876
Titre abrégé: J Transl Med
Pays: England
ID NLM: 101190741
Informations de publication
Date de publication:
19 06 2019
19 06 2019
Historique:
received:
06
03
2019
accepted:
09
06
2019
entrez:
21
6
2019
pubmed:
21
6
2019
medline:
1
7
2020
Statut:
epublish
Résumé
In our recent clinical trial, increased peripheral concentrations of pro-inflammatory molecular mediators were determined in complex regional pain syndrome (CRPS) patients. After 3 months adjunctive unilateral, selective L4 dorsal root ganglion stimulation (L4-DRG Blood samples were collected from 12 CRPS patients for whole-transcriptome profiling in order to assay 18,845 inflammation-associated genes from frozen blood at baseline and after 3 months L4-DRG Pathway enrichment analyses tools (GOrilla™ and PANTHER™) showed predominant involvement of inflammation mediated by chemokines/cytokines and gonadotropin releasing hormone receptor pathways. Further, screening of differentially regulated genes showed changes in innate immune response related genes. Transcriptomic analysis showed that 21 genes (predominantly immunoinflammatory) were significantly changed after L4-DRG In our sub-group analysis of L4-DRG
Sections du résumé
BACKGROUND
In our recent clinical trial, increased peripheral concentrations of pro-inflammatory molecular mediators were determined in complex regional pain syndrome (CRPS) patients. After 3 months adjunctive unilateral, selective L4 dorsal root ganglion stimulation (L4-DRG
METHODS
Blood samples were collected from 12 CRPS patients for whole-transcriptome profiling in order to assay 18,845 inflammation-associated genes from frozen blood at baseline and after 3 months L4-DRG
RESULTS
Pathway enrichment analyses tools (GOrilla™ and PANTHER™) showed predominant involvement of inflammation mediated by chemokines/cytokines and gonadotropin releasing hormone receptor pathways. Further, screening of differentially regulated genes showed changes in innate immune response related genes. Transcriptomic analysis showed that 21 genes (predominantly immunoinflammatory) were significantly changed after L4-DRG
CONCLUSIONS
In our sub-group analysis of L4-DRG
Identifiants
pubmed: 31217010
doi: 10.1186/s12967-019-1952-x
pii: 10.1186/s12967-019-1952-x
pmc: PMC6585082
doi:
Substances chimiques
Biomarkers
0
Cytokines
0
Inflammation Mediators
0
Banques de données
DRKS
['DRKS00011267']
Types de publication
Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
205Références
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