Co-expression of carcinoembryonic antigen-related cell adhesion molecule 6 and 8 inhibits proliferation and invasiveness of breast carcinoma cells.
Adult
Aged
Aged, 80 and over
Antigens, CD
/ metabolism
Apoptosis
Biomarkers, Tumor
/ metabolism
Bone Neoplasms
/ metabolism
Breast Neoplasms
/ metabolism
Cell Adhesion Molecules
/ metabolism
Cell Proliferation
Female
Follow-Up Studies
GPI-Linked Proteins
/ metabolism
Gene Expression Regulation, Neoplastic
Humans
Lung Neoplasms
/ metabolism
Middle Aged
Neoplasm Invasiveness
Prognosis
Tumor Cells, Cultured
Breast cancer
CEACAM6
CEACAM8
Cancer metastasis
Protein–protein interaction
Journal
Clinical & experimental metastasis
ISSN: 1573-7276
Titre abrégé: Clin Exp Metastasis
Pays: Netherlands
ID NLM: 8409970
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
21
02
2019
accepted:
18
06
2019
pubmed:
22
6
2019
medline:
11
4
2020
entrez:
22
6
2019
Statut:
ppublish
Résumé
The carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 6 and CEACAM8 form heterodimers and exert their effects. Therefore, we examined the effects of CEACAM6 and CEACAM8 co-expression in breast cancer. We first studied CEACAM6/8 expression using immunohistochemistry in 109 patients with breast cancer. We then established MCF-7 cells that were stably transfected with CEACAM8 and studied cell proliferation, invasion and adhesion. The number of CEACAM6 and CEACAM8 double-positive breast carcinoma cells significantly increased in patients with low histopathological grade and stage. Proximity ligation assay (PLA) confirmed high CEACAM6/8 expression in MCF-7 cells. CEACAM6/8 expression promoted the adhesion of MCF-7 cells to endothelial cell monolayers but inhibited their invasion and proliferation. Furthermore, CEACAM6 status in carcinoma cells was significantly higher in bone than in lung metastases. CEACAM6/8 expression is associated with the inhibition of vascular invasion and cell proliferation. CEACAM6 expression was also considered to be involved in bone metastases of breast cancer. This is the first study to demonstrate the possible role of CEACAM6/8 heterodimer and CEACAM6 expression in breast cancer patients.
Identifiants
pubmed: 31222613
doi: 10.1007/s10585-019-09981-2
pii: 10.1007/s10585-019-09981-2
doi:
Substances chimiques
Antigens, CD
0
Biomarkers, Tumor
0
CEACAM6 protein, human
0
CEACAM8 protein, human
0
Cell Adhesion Molecules
0
GPI-Linked Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
423-432Références
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