Alterations in the expression of EMT-related proteins claudin-1, claudin-4 and claudin-7, E-cadherin, TWIST1 and ZEB1 in oral lichen planus.
Antigens, CD
/ genetics
Cadherins
/ genetics
Carcinoma, Squamous Cell
/ genetics
Case-Control Studies
Claudin-1
/ metabolism
Claudin-4
/ metabolism
Claudins
/ genetics
Epithelial-Mesenchymal Transition
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Lichen Planus, Oral
/ genetics
Mouth Neoplasms
/ genetics
Nuclear Proteins
/ genetics
Twist-Related Protein 1
/ genetics
Zinc Finger E-box-Binding Homeobox 1
/ genetics
epithelial-to-mesenchymal transition
oral lichen planus
Journal
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
ISSN: 1600-0714
Titre abrégé: J Oral Pathol Med
Pays: Denmark
ID NLM: 8911934
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
04
03
2019
revised:
05
06
2019
accepted:
13
06
2019
pubmed:
23
6
2019
medline:
10
1
2020
entrez:
23
6
2019
Statut:
ppublish
Résumé
Oral lichen planus (OLP) is a chronic T-cell-mediated inflammatory disease, which is associated with increased risk of developing oral squamous cell carcinoma. Epithelial-to-mesenchymal transition is a physiological phenomenon occurring during growth and organogenesis, but it has also an important role in tumorigenesis. In the present work, we studied the expression of known epithelial-to-mesenchymal transition markers in oral lichen planus. In total, 54 oral lichen planus and 22 control samples were analyzed for epithelial-to-mesenchymal transition markers. Samples were immunohistochemically stained for claudin-1, claudin-4 and claudin-7, cadherin-1 (E-cadherin), Twist-related protein 1 (TWIST1) and zinc finger E-box-binding homeobox 1 (ZEB1). The expression of claudin-1, claudin-4 and E-cadherin was significantly weaker in oral lichen planus epithelium compared to controls (P < 0.001). The quantity of claudin-7-expressing cells (P < 0.001) and claudin-7 staining intensity (P < 0.05) in the stroma was greater in lichen planus than in control samples. TWIST1 and ZEB1 stainings were negative in the epithelium in both lichen planus and controls. The number of TWIST1-expressing cells in the stroma was higher in lichen planus than in controls (P < 0.001). There was a statistically significant difference in ZEB1 staining intensity in the stroma between lichen planus and control samples (P < 0.05). The data indicate that the expression of claudin-1, claudin-4 and E-cadherin is decreased in oral lichen planus. This may lead to disturbance in epithelial tight junctions, cell-cell connections and epithelial permeability, contributing to oral lichen planus pathogenesis. Based on the present study, the role of TWIST1 and ZEB1 in oral lichen planus remains unclear.
Sections du résumé
BACKGROUND
BACKGROUND
Oral lichen planus (OLP) is a chronic T-cell-mediated inflammatory disease, which is associated with increased risk of developing oral squamous cell carcinoma. Epithelial-to-mesenchymal transition is a physiological phenomenon occurring during growth and organogenesis, but it has also an important role in tumorigenesis. In the present work, we studied the expression of known epithelial-to-mesenchymal transition markers in oral lichen planus.
METHODS
METHODS
In total, 54 oral lichen planus and 22 control samples were analyzed for epithelial-to-mesenchymal transition markers. Samples were immunohistochemically stained for claudin-1, claudin-4 and claudin-7, cadherin-1 (E-cadherin), Twist-related protein 1 (TWIST1) and zinc finger E-box-binding homeobox 1 (ZEB1).
RESULTS
RESULTS
The expression of claudin-1, claudin-4 and E-cadherin was significantly weaker in oral lichen planus epithelium compared to controls (P < 0.001). The quantity of claudin-7-expressing cells (P < 0.001) and claudin-7 staining intensity (P < 0.05) in the stroma was greater in lichen planus than in control samples. TWIST1 and ZEB1 stainings were negative in the epithelium in both lichen planus and controls. The number of TWIST1-expressing cells in the stroma was higher in lichen planus than in controls (P < 0.001). There was a statistically significant difference in ZEB1 staining intensity in the stroma between lichen planus and control samples (P < 0.05).
CONCLUSIONS
CONCLUSIONS
The data indicate that the expression of claudin-1, claudin-4 and E-cadherin is decreased in oral lichen planus. This may lead to disturbance in epithelial tight junctions, cell-cell connections and epithelial permeability, contributing to oral lichen planus pathogenesis. Based on the present study, the role of TWIST1 and ZEB1 in oral lichen planus remains unclear.
Substances chimiques
Antigens, CD
0
CDH1 protein, human
0
CLDN1 protein, human
0
CLDN4 protein, human
0
CLDN7 protein, human
0
Cadherins
0
Claudin-1
0
Claudin-4
0
Claudins
0
Nuclear Proteins
0
TWIST1 protein, human
0
Twist-Related Protein 1
0
ZEB1 protein, human
0
Zinc Finger E-box-Binding Homeobox 1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
735-744Subventions
Organisme : Sigrid Juselius Foundation (S.P.-S.)
Organisme : The Finnish Dental Society Apollonia (L.H.)
Organisme : Plandent Oy and Planmeca Oy (L.H.)
Informations de copyright
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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