Self-Organization of Minimal Anaphase Spindle Midzone Bundles.
Cytosim
anaphase
computer simulation
in vitro reconstitution
kinesin
microtubule
mitotic spindle
motor protein
self-organization
spindle midzone
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
08 07 2019
08 07 2019
Historique:
received:
22
01
2019
revised:
15
04
2019
accepted:
20
05
2019
pubmed:
25
6
2019
medline:
7
7
2020
entrez:
25
6
2019
Statut:
ppublish
Résumé
In anaphase spindles, antiparallel microtubules associate to form tight midzone bundles, as required for functional spindle architecture and correct chromosome segregation. Several proteins selectively bind to these overlaps to control cytokinesis. How midzone bundles assemble is poorly understood. Here, using an in vitro reconstitution approach, we demonstrate that minimal midzone bundles can reliably self-organize in solution from dynamic microtubules, the microtubule crosslinker PRC1, and the motor protein KIF4A. The length of the central antiparallel overlaps in these microtubule bundles is similar to that observed in cells and is controlled by the PRC1/KIF4A ratio. Experiments and computer simulations demonstrate that minimal midzone bundle formation results from promoting antiparallel microtubule crosslinking, stopping microtubule plus-end dynamicity, and motor-driven midzone compaction and alignment. The robustness of this process suggests that a similar self-organization mechanism may contribute to the reorganization of the spindle architecture during the metaphase to anaphase transition in cells.
Identifiants
pubmed: 31231047
pii: S0960-9822(19)30619-0
doi: 10.1016/j.cub.2019.05.049
pmc: PMC6616649
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2120-2130.e7Subventions
Organisme : Wellcome Trust
ID : FC001163
Pays : United Kingdom
Organisme : European Research Council
ID : 323042
Pays : International
Organisme : Medical Research Council
ID : FC001163
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : FC001163
Pays : United Kingdom
Organisme : Cancer Research UK
ID : FC001163
Pays : United Kingdom
Informations de copyright
Copyright © 2019 Francis Crick Institute. Published by Elsevier Ltd.. All rights reserved.
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