Specific basic patch-dependent multimerization of Saccharomyces cerevisiae ORC on single-stranded DNA promotes ATP hydrolysis.
AAA+
ATPase
DNA replication
intrinsically disordered region
origin recognition complex
protein multimerization
single-stranded DNA
Journal
Genes to cells : devoted to molecular & cellular mechanisms
ISSN: 1365-2443
Titre abrégé: Genes Cells
Pays: England
ID NLM: 9607379
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
17
05
2019
revised:
12
06
2019
accepted:
18
06
2019
pubmed:
25
6
2019
medline:
10
1
2020
entrez:
25
6
2019
Statut:
ppublish
Résumé
Replication initiation at specific genomic loci dictates precise duplication and inheritance of genetic information. In eukaryotic cells, ATP-bound origin recognition complexes (ORCs) stably bind to double-stranded (ds) DNA origins to recruit the replicative helicase onto the origin DNA. To achieve these processes, an essential region of the origin DNA must be recognized by the eukaryotic origin sensor (EOS) basic patch within the disordered domain of the largest ORC subunit, Orc1. Although ORC also binds single-stranded (ss) DNA in an EOS-independent manner, it is unknown whether EOS regulates ORC on ssDNA. We found that, in budding yeast, ORC multimerizes on ssDNA in vitro independently of adenine nucleotides. We also found that the ORC multimers form in an EOS-dependent manner and stimulate the ORC ATPase activity. An analysis of genomics data supported the idea that ORC-ssDNA binding occurs in vivo at specific genomic loci outside of replication origins. These results suggest that EOS function is differentiated by ORC-bound ssDNA, which promotes ORC self-assembly and ATP hydrolysis. These mechanisms could modulate ORC activity at specific genomic loci and could be conserved among eukaryotes.
Substances chimiques
DNA, Single-Stranded
0
Origin Recognition Complex
0
Saccharomyces cerevisiae Proteins
0
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
608-618Subventions
Organisme : Shinnihon Foundation of Advanced Medical Treatment Research
Organisme : Naito Foundation
Organisme : Japan Society for the Promotion of Science
ID : JP15K18504
Organisme : Japan Society for the Promotion of Science
ID : JP17H03656
Organisme : Japan Society for the Promotion of Science
ID : JP17K07338
Organisme : Japan Society for the Promotion of Science
ID : JP26291004
Informations de copyright
© 2019 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
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