A sensitive and radiolabeling-free method for pseudouridine detection.

DNA, Catalytic / chemistry Pseudouridine / analysis RNA, Fungal / analysis Saccharomyces cerevisiae / metabolism
DNAzyme Pseudouridine (Ψ) Rolling circle amplification (RCA)

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
15 09 2019
Historique:
received: 16 05 2019
revised: 25 06 2019
accepted: 26 06 2019
pubmed: 1 7 2019
medline: 20 3 2020
entrez: 1 7 2019
Statut: ppublish

Résumé

Existing methodologies for detecting Pseudouridine (Ψ) mostly use CMCT labeling or radiolabeling. Described herein is a sensitive and quantitative method for Ψ detection that does not need this labelling. This approach combines the selectivity of a 10-23 DNAzyme, which can distinguish Ψ from uridine (U), with rolling circle amplification (RCA) to increase the sensitivity of the assay.

Identifiants

DOI: 10.1016/j.ab.2019.113350 PMID: 31255565
pubmed: 31255565
pii: S0003-2697(19)30470-1
doi: 10.1016/j.ab.2019.113350
pii:
doi:

Substances chimiques

DNA, Catalytic 0
RNA, Fungal 0
Pseudouridine 1445-07-4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

113350

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Wei Li (W)

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, 430072, PR China.

Fang Wang (F)

Wuhan University School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China.

Yi Chen (Y)

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, 430072, PR China.

Xiaocheng Weng (X)

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, 430072, PR China. Electronic address: xcweng@whu.edu.cn.

Xiang Zhou (X)

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, 430072, PR China.

Articles similaires

Saccharomyces cerevisiae whole cell biotransformation for the production of aldehyde flavors and fragrances.

Nazreen V M Abdul Muthaliff, Yao Zong Ng, Wei Mei Guo et al.
1.00
Saccharomyces cerevisiae Aldehydes Biotransformation Flavoring Agents Lipoxygenase

The dual life of disordered lysine-rich domains of snoRNPs in rRNA modification and nucleolar compaction.

Carine Dominique, Nana Kadidia Maiga, Alfonso Méndez-Godoy et al.
1.00
Saccharomyces cerevisiae Lysine Cell Nucleolus RNA, Ribosomal Saccharomyces cerevisiae Proteins

Minimisation of metabolic networks defines a new functional class of genes.

Giorgio Jansen, Tanda Qi, Vito Latora et al.
1.00
Metabolic Networks and Pathways Saccharomyces cerevisiae Computational Biology Synthetic Biology Computer Simulation

Effects of Pichia manshurica yeast supplementation on ruminal fermentation, nutrient degradability, and greenhouse gas emissions in aflatoxin B1 contaminated diets.

Yosra Soltan, Amr Morsy, Mahmoud Elazab et al.
1.00
Animals Rumen Fermentation Animal Feed Diet

Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN catalytique › A sensitive and radiolabeling-free method for...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléosides › Ribonucléosides › Uridine › Pseudouridine › A sensitive and radiolabeling-free method for...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN fongique › A sensitive and radiolabeling-free method for...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › A sensitive and radiolabeling-free method for...