Transcriptomes of antigen presenting cells in human thymus.
Antigen Presentation
/ genetics
Antigens, Surface
/ genetics
B-Lymphocytes
/ cytology
DNA-Binding Proteins
/ genetics
Dendritic Cells
/ cytology
Epithelial Cells
/ cytology
Gene Expression Profiling
HLA Antigens
/ classification
Humans
Immunophenotyping
Infant
Infant, Newborn
Interleukin-3 Receptor alpha Subunit
/ genetics
Male
Primary Cell Culture
Thrombomodulin
Thymus Gland
/ cytology
Transcription Factors
/ genetics
Transcriptome
/ immunology
AIRE Protein
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
17
11
2018
accepted:
11
06
2019
entrez:
2
7
2019
pubmed:
2
7
2019
medline:
19
2
2020
Statut:
epublish
Résumé
Antigen presenting cells (APCs) in the thymus play an essential role in the establishment of central tolerance, i.e. the generation of a repertoire of functional and self-tolerant T cells to prevent autoimmunity. In this study, we have compared the transcriptomes of four primary APCs from human thymus (mTECs, CD19+ B cells, CD141+ and CD123+ DCs). We investigated a set of genes including the HLA genes, genes encoding transcriptional regulators and finally, tissue-enriched genes, i.e, genes with a five-fold higher expression in a particular human tissue. We show that thymic CD141+ DCs express the highest levels of all classical HLA genes and 67% (14/21) of the HLA class I and II pathway genes investigated in this study. CD141+ DCs also expressed the highest levels of the transcriptional regulator DEAF1, whereas AIRE and FEZF2 expression were mainly found in primary human mTECs. We found expression of "tissue enriched genes" from the Human Protein Atlas (HPA) in all four APC types, but the mTECs were clearly dominating in the number of uniquely expressed tissue enriched genes (20% in mTECs, 7% in CD19+ B cells, 4% in CD123+ DCs and 2% in CD141+ DCs). The tissue enriched genes also overlapped with reported human autoantigens. This is, to our knowledge, the first study that performs RNA sequencing of mTECs, CD19+ B cells, CD141+ and CD123+ DCs isolated from the same individuals and provides insight into the transcriptomes of these human thymic APCs.
Identifiants
pubmed: 31261375
doi: 10.1371/journal.pone.0218858
pii: PONE-D-18-33185
pmc: PMC6602790
doi:
Substances chimiques
Antigens, Surface
0
DEAF1 protein, human
0
DNA-Binding Proteins
0
FEZF2 protein, human
0
HLA Antigens
0
IL3RA protein, human
0
Interleukin-3 Receptor alpha Subunit
0
THBD protein, human
0
Thrombomodulin
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0218858Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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