Microbial Composition of the Human Nasopharynx Varies According to Influenza Virus Type and Vaccination Status.
Adolescent
Adult
Aged
Aged, 80 and over
Bacteria
/ classification
Child
Child, Preschool
Cluster Analysis
Community-Acquired Infections
/ immunology
DNA, Ribosomal
/ chemistry
Female
Humans
Infant
Influenza A virus
/ growth & development
Influenza B virus
/ growth & development
Influenza Vaccines
/ administration & dosage
Influenza, Human
/ prevention & control
Male
Microbiota
Middle Aged
Nasopharynx
/ microbiology
Phylogeny
RNA, Ribosomal, 16S
/ genetics
Sequence Analysis, DNA
Young Adult
16S RNA sequencing
influenza virus
microbiome
vaccination
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
02 07 2019
02 07 2019
Historique:
entrez:
4
7
2019
pubmed:
4
7
2019
medline:
24
4
2020
Statut:
epublish
Résumé
Factors that contribute to enhanced susceptibility to severe bacterial disease after influenza virus infection are not well defined but likely include the microbiome of the respiratory tract. Vaccination against influenza, while having variable effectiveness, could also play a role in microbial community stability. We collected nasopharyngeal samples from 215 individuals infected with influenza A/H3N2 or influenza B virus and profiled the microbiota by target sequencing of the 16S rRNA gene. We identified signature taxonomic groups by performing linear discriminant analysis and effective size comparisons (LEfSe) and defined bacterial community types using Dirichlet multinomial mixture (DMM) models. Influenza infection was shown to be significantly associated with microbial composition of the nasopharynx according to the virus type and the vaccination status of the patient. We identified four microbial community types across the combined cohort of influenza patients and healthy individuals with one community type most representative of the influenza virus-infected group. We also identified microbial taxa for which relative abundance was significantly higher in the unvaccinated elderly group; these taxa include species known to be associated with pneumonia.
Identifiants
pubmed: 31266874
pii: mBio.01296-19
doi: 10.1128/mBio.01296-19
pmc: PMC6606809
pii:
doi:
Substances chimiques
DNA, Ribosomal
0
Influenza Vaccines
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R21 AI124141
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI111598
Pays : United States
Informations de copyright
Copyright © 2019 Ding et al.
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