Biochemical characterization and kinetic/thermodynamic study of Aspergillus tamarii URM4634 β-fructofuranosidase with transfructosylating activity.
Aspergillus
fructo-oligosaccharides
kinetic and thermodynamic parameters
solid state fermentation
β-fructofuranosidase
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
02
05
2019
revised:
19
06
2019
accepted:
01
07
2019
pubmed:
4
7
2019
medline:
7
7
2020
entrez:
4
7
2019
Statut:
ppublish
Résumé
This study reports on the biochemical characterization as well as the kinetic and thermodynamic study of Aspergillus tamarii URM4634 β-fructofuranosidase (FFase) with transfructosylating activity. Conditions for FFase activity were optimized by means of a central composite rotational design using pH and temperature as the independent variables, while residual activity tests carried out in the temperature range of 45-65°C enabled us to investigate FFase thermostability and estimate the kinetic and thermodynamic parameters of enzyme denaturation. Optimal conditions for sucrose hydrolysis and fructosyl transfer catalyzed by crude FFase were 50°C, and pH 6.0 and 7.4, respectively. The thermodynamic properties of irreversible enzyme inactivation were found to be activation energy of 293.1 kJ mol
Substances chimiques
Fructose
30237-26-4
beta-Fructofuranosidase
EC 3.2.1.26
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2879Informations de copyright
© 2019 American Institute of Chemical Engineers.
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