Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel-derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid.


Journal

Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280

Informations de publication

Date de publication:
Oct 2019
Historique:
received: 23 11 2018
revised: 10 06 2019
accepted: 24 06 2019
pubmed: 10 7 2019
medline: 18 12 2019
entrez: 10 7 2019
Statut: ppublish

Résumé

To assess the ability of various newly isolated or belonging in official collections yeast strains to convert biodiesel-derived glycerol (Gly) into added-value compounds. Ten newly isolated yeast strains belonging to Debaryomyces sp., Naganishia uzbekistanensis, Rhodotorula sp. and Yarrowia lipolytica, isolated from fishes, metabolized Gly under nitrogen limitation. The aim of the study was to identify potential newly isolated microbial candidates that could produce single-cell oil (SCO), endopolysaccharides and polyols when these micro-organisms were grown on biodiesel-derived Gly. As controls producing SCO and endopolysaccharides were the strains Rhodotorula glutinis NRRL YB-252 and Cryptococcus curvatus NRRL Y-1511. At initial Gly (Gly Newly isolated, food-derived and non-previously studied yeast isolates converted biodiesel-derived Gly into several added-value metabolites. Alternative ways of crude Gly valorization through yeast fermentations were provided and added-value compounds were synthesized.

Identifiants

pubmed: 31286622
doi: 10.1111/jam.14373
doi:

Substances chimiques

Biofuels 0
Fungal Polysaccharides 0
Lipids 0
Polymers 0
polyol 0
Glycerol PDC6A3C0OX

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1080-1100

Subventions

Organisme : Ministry of National Education and Religious Affairs, Greece
ID : Acronym: BIO4OIL, project code 2359
Organisme : S&T
ID : 2013-2015

Informations de copyright

© 2019 The Society for Applied Microbiology.

Références

André, A., Chatzifragkou, A., Diamantopoulou, P., Sarris, D., Philippoussis, A., Galiotou-Panayotou, M., Komaitis, M. and Papanikolaou, S. (2009) Biotechnological conversions of bio-diesel derived crude glycerol by Yarrowia lipolytica strains. Eng Life Sci 9, 468-478.
Argyri, A.A., Doulgeraki, A.I., Blana, V.A., Panagou, E.Z. and Nychas, G.-J.E. (2011) Potential of a simple HPLC-based approach for the identification of the spoilage status of minced beef stored at various temperatures and packaging systems. Int J Food Microbiol 150, 25-33.
Arous, F., Triantaphyllidou, I.-E., Mechichi, T., Azabou, S., Nasri, M. and Aggelis, G. (2015) Lipid accumulation in the new oleaginous yeast Debaryomyces etchellsii correlates with ascosporogenesis. Biomass Bioenerg 80, 307-315.
Arous, F., Frikha, F., Triantaphyllidou, I.-E., Aggelis, G., Nasri, M. and Mechichi, T. (2016a) Potential utilization of agro-industrial wastewaters for lipid production by the oleaginous yeast Debaryomyces etchellsii. J Clean Prod 133, 899-909.
Arous, F., Mechichi, T., Nasri, M. and Aggelis, G. (2016b) Fatty acid biosynthesis during the life cycle of Debaryomyces etchellsii. Microbiology UK 162, 1080-1090.
Arous, F., Azabou, S., Triantaphyllidou, I.-E., Aggelis, G., Jaouani, A., Nasri, M. and Mechichi, T. (2017) Newly isolated yeasts from Tunisian microhabitats: lipid accumulation and fatty acid composition. Eng Life Sci 17, 226-236.
Athenaki, M., Gardeli, C., Tchakouteu, S.S., Diamantopoulou, P., Sarris, D., Philippoussis, A. and Papanikolaou, S. (2018) Lipids from yeasts and fungi: physiology, production and analytical considerations. J Appl Microbiol 124, 336-367.
Ayadi, I., Kamoun, O., Trigui-Lahiani, H., Hdiji, A., Gargouri, A., Belghith, H. and Guerfali, M. (2016) Single cell oil production from a newly isolated Candida viswanathii Y-E4 and agro-industrial by-products valorization. J Ind Microbiol Biotechnol 43, 901-914.
Ayadi, I., Belghith, H., Gargouri, A. and Guerfali, M. (2018) Screening of new oleaginous yeasts for single cell oil production, hydrolytic potential exploitation and agro-industrial by-products valorization. Process Saf Environ Prot 119, 104-114.
Baleiras Couto, M.M., Eijsma, B., Hofstra, H., Huis in’t Veld, J.H.J. and van der Vossen, J.M.B.M. (1996) Evaluation of molecular techniques to assign genetic diversity among Saccharomyces cerevisiae strains. Appl Environ Microbiol 62, 41-46.
Bellou, S., Moustogianni, A., Makri, A. and Aggelis, G. (2012) Lipids containing polyunsaturated fatty acids synthesized by Zygomycetes grown on glycerol. App Biochem Biotechnol 166, 146-158.
Bellou, S., Triantaphyllidou, I.E., Aggeli, D., Elazzazy, A.M., Baeshen, M.N. and Aggelis, G. (2016) Microbial oils as food additives: recent approaches for improving microbial oil production and its polyunsaturated fatty acid content. Curr Opin Biotechnol 37, 24-35.
Carota, E., Crognale, S., D’Annibale, A. and Petruccioli, M. (2018) Bioconversion of agro-industrial waste into microbial oils by filamentous fungi. Process Saf Environ Prot 117, 143-151.
Carsanba, E., Papanikolaou, S. and Erten, H. (2018) Production of oils and fats by oleaginous microorganisms with an emphasis given to the potential of the nonconventional yeast Yarrowia lipolytica. Crit Rev Biotechnol 38, 1230-1243.
Chatzifragkou, A., Makri, A., Belka, A., Bellou, S., Mavrou, M., Mastoridou, M., Mystrioti, P., Onjaro, G. et al. (2011) Biotechnological conversions of biodiesel derived waste glycerol by yeast and fungal species. Energy 36, 1097-1108.
Crognale, S., D'Annibale, A., Federici, F., Fenice, M., Quaratino, D. and Petruccioli, M. (2006) Olive oil mill wastewater valorization by fungi. J Chem Technol Biotechnol 81, 1547-1555.
Cui, Y., Blackburn, J.W. and Liang, Y. (2012) Fermentation optimization for the production of lipid by Cryptococcus curvatus: use of response surface methodology. Biomass Bioenerg 47, 410-417.
Dedyukhina, E.G., Chistyakova, T.I., Mironov, A.A., Kamzolova, S.V., Morgunov, I.G. and Vainshtein, M.B. (2014) Arachidonic acid synthesis from biodiesel-derived waste by Mortierella alpina. Eur J Lipid Sci Technol 116, 429-437.
Diamantopoulou, P., Papanikolaou, S., Katsarou, E., Komaitis, M., Aggelis, G. and Philippoussis, A. (2012) Mushroom polysaccharides and lipids synthesized in liquid agitated and static cultures. Part II: Study of Volvariella volvacea. Appl Biochem Biotechnol 167, 1890-1906.
Dien, B., Kurtzman, C., Saha, B.C. and Bothast, R.J. (1996) Screening for L-arabinose fermenting yeasts. Appl Biochem Biotechnol 57, 223-242.
Dobrowolski, A., Mituła, P., Rymowicz, W. and Mirończuk, A.M. (2016) Efficient conversion of crude glycerol from various industrial wastes into single cell oil by yeast Yarrowia lipolytica. Bioresour Technol 207, 237-243.
Egermeier, M., Russmayer, H., Sauer, M. and Marx, H. (2017) Metabolic flexibility of Yarrowia lipolytica growing on glycerol. Front Microbiol 8, 49.
Escalante, J., Caminal, G., Figueredo, M. and De Mas, C. (1990) Production of arabitol from glucose by Hansenula polymorpha. J Ferment Bioeng 70, 228-231.
Esteve-Zarzoso, B., Belloch, C., Uruburu, F. and Querol, A. (1999) Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Int J Syst Bacteriol 49, 329-337.
Fakas, S., Papanikolaou, S., Galiotou-Panayotou, M., Komaitis, M. and Aggelis, G. (2008) Organic nitrogen of tomato waste hydrolysate enhances glucose uptake and lipid accumulation in Cunninghamella echinulata. J Appl Microbiol 105, 1062-1070.
Fernández-Espinar, M.T., Esteve-Zarzoso, B., Querol, A. and Barrio, E. (2000) RFLP analysis of the ribosomal internal transcribed spacers and the 5.8S rRNA gene region of the genus Saccharomyces: a fast method for species identification and differentiation of flor yeasts. Antonie Van Leeuwenhoek 78, 87-97.
Fontanille, P., Kumar, V., Christophe, G., Nouaille, R. and Larroche, C. (2012) Bioconversion of volatile fatty acids into lipids by the oleaginous yeast Yarrowia lipolytica. Bioresour Technol 114, 443-449.
Fu, G.Y., Lu, Y., Chi, Z., Liu, G.L., Zhao, S.F., Jiang, H. and Chi, Z.M. (2016) Cloning and characterization of a pyruvate carboxylase gene from Penicillium rubens and overexpression of the gene in the yeast Yarrowia lipolytica for enhanced citric acid production. Marine Biotechnol 18, 1-14.
Gadanho, M. and Sampaio, J.P. (2005) Occurrence and diversity of yeasts in the mid-Atlantic ridge hydrothermal fields near the Azores Archipelago. Microb Ecol 50, 408-417.
Gardeli, C., Athenaki, M., Xenopoulos, E., Mallouchos, A., Koutinas, A.A., Aggelis, G. and Papanikolaou, S. (2017) Lipid production and characterization by Mortierella (Umbelopsis) isabellina cultivated on lignocellulosic sugars. J Appl Microbiol 123, 1461-1477.
Gil-Serna, J., Patino, B., Gonzalez-Jaen, M.T. and Vazquez, C. (2011) Mechanisms involved in reduction of ochratoxin A produced by Aspergillus westerdijkiae using Debaryomyces hansenii CYC 1244. Int J Food Microbiol 151, 113-118.
Gram, L. and Huss, H.H. (1996) Microbiological spoilage of fish and fish products. Int J Food Microbiol 33, 121-137.
Guillamón, J.M., Sabaté, J., Barrio, E., Cano, J. and Querol, A. (1998) Rapid identification of wine yeast species based on RFLP analysis of the ribosomal internal transcribed spacer (ITS) region. Arch Microbiol 169, 387-392.
Kachrimanidou, V., Kopsahelis, N., Chatzifragkou, A., Papanikolaou, S., Yanniotis, S., Kookos, I.K. and Koutinas, A.A. (2013) Utilisation of by-products from sunflower-based biodiesel production processes for the production of fermentation feedstock. Waste Biomass Valor 4, 529-537.
Kamzolova, S.V., Fatykhova, A.R., Dedyukhina, E.G., Anastassiadis, S.G., Golovchenko, N.P. and Morgunov, I.G. (2011) Citric acid production by yeast grown on glycerol-containing waste from biodiesel industry. Food Technol Biotechnol 49, 65-74.
Koganti, S. and Ju, L.K. (2013) Debaryomyces hansenii fermentation for arabitol production. Biochem Eng J 79, 112-119.
Koganti, S., Kuo, T.M., Kurtzman, C.P., Smith, N. and Ju, L.K. (2011) Production of arabitol from glycerol: strain screening and study of factors affecting production yield. Appl Microbiol Biotechnol 90, 257-267.
Kordowska-Wiater, M. (2015) Production of arabitol by yeasts: current status and future prospects. J Appl Microbiol 119, 303-314.
Kordowska-Wiater, M., Targoński, Z. and Jarosz, A. (2008) Biotransformation of L-arabinose to arabitol by yeasts from genera Pichia and Candida. Biotechnologia 1, 177-188.
Kordowska-Wiater, M., Kuzdraliński, A., Czernecki, T., Targoński, Z., Frąc, M. and Oszust, K. (2017) The production of arabitol by a novel plant yeast isolate Candida parapsilosis 27RL-4. Open Life Sci 12, 326-336.
Kumdam, H., Murthy, S.N. and Gummadi, S.N. (2013) Production of ethanol and arabitol by Debaryomyces nepalensis: influence of process parameters. AMB Express 3, 23.
Kurtzman, C.P., Fell, J.W. and Boekhout, T. (2011) The Yeasts. A Taxonomic Study (5th edn). Amsterdam: Elsevier.
Li, M., Liu, G.L., Chi, Z. and Chi, Z.M. (2010) Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TJY15a. Biomass Bioenerg 34, 101-107.
Li, C., Yang, X., Gao, S., Wang, H. and Lin, C.S.K. (2017) High efficiency succinic acid production from glycerol via in situ fibrous bed bioreactor with an engineered Yarrowia lipolytica. Bioresour Technol 225, 9-16.
Li, C., Gao, S., Yang, X. and Lin, C.S.K. (2017) Green and sustainable succinic acid production from crude glycerol by engineered Yarrowia lipolytica via agricultural residue based in situ fibrous bed bioreactor. Bioresour Technol 249, 612-619.
Liang, Y.N., Cui, Y., Trushenski, J. and Blackburn, J.W. (2010) Converting crude glycerol derived from yellow grease to lipids through yeast fermentation. Bioresour Technol 101, 7581-7586.
Lin, C.C.S. and Fung, D.Y. (1987) Conventional and rapid methods for yeast identification. Crit Rev Microbiol 14, 273-289.
Liu, J., Sui, Y., Wisniewski, M., Droby, S. and Liu, Y. (2013a) Review: utilization of antagonistic yeasts to manage postharvest fungal diseases of fruit. Int J Food Microbiol 167, 153-160.
Liu, X.Y., Chi, Z., Liu, G.L., Madzak, C. and Chi, Z.M. (2013b) Both decrease in ACL1 gene expression and increase in ICL1 gene expression in marine-derived yeast Yarrowia lipolytica expressing INU1 gene enhance citric acid production from inulin. Marine Biotechnol 15, 26-36.
Loman, A.A., Islam, S.M.M. and Ju, L.K. (2018) Production of arabitol from enzymatic hydrolysate of soybean flour by Debaryomyces hansenii fermentation. Appl Microbiol Biotechnol 102, 641-653.
Maina, S., Pateraki, C., Kopsahelis, N., Paramithiotis, S., Drosinos, E.H., Papanikolaou, S. and Koutinas, A.A. (2017) Microbial oil production from various carbon sources by newly isolated oleaginous yeasts. Eng Life Sci 17, 333-344.
Makri, A., Fakas, S. and Aggelis, G. (2010) Metabolic activities of biotechnological interest in Yarrowia lipolytica grown on glycerol in repeated batch cultures. Bioresour Technol 101, 2351-2358.
Meesters, P.A.E.P., Huijberts, G.N.M. and Eggink, G. (1996) High cell density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source. Appl Microbiol Biotechnol 45, 575-579.
Mirończuk, M.A., Furgala, I., Rakicka, M. and Rymowicz, W. (2014) Enhanced production of erythritol by Yarrowia lipolytica on gycerol in repeated batch cultures. J Ind Microbiol Biotechnol 41, 57-64.
Morgunov, I.G., Kamzolova, S.V. and Lunina, J.N. (2013) The citric acid production from raw glycerol by Yarrowia lipolytica yeast and its regulation. Appl Microbiol Biotechnol 97, 7387-7397.
Moser, B.R. (2009) Biodiesel production, properties, and feedstocks. In Vitro Cell Develop Biol Plant 45, 229-266.
Papanikolaou, S. and Aggelis, G. (2002) Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture. Bioresour Technol 82, 43-49.
Papanikolaou, S. and Aggelis, G. (2011a) Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production. Eur J Lipid Sci Technol 113, 1031-1051.
Papanikolaou, S. and Aggelis, G. (2011b) Lipids of oleaginous yeasts. Part II: Technology and potential applications. Eur J Lipid Sci Technol 113, 1052-1073.
Papanikolaou, S., Muniglia, L., Chevalot, I., Aggelis, G. and Marc, I. (2002) Yarrowia lipolytica as a potential producer of citric acid from raw glycerol. J Appl Microbiol 92, 737-744.
Papanikolaou, S., Sarantou, S., Komaitis, M. and Aggelis, G. (2004) Repression of reserve lipid turnover in Cunninghamella echinulata and Mortierella isabellina cultivated in multiple-limited media. J Appl Microbiol 97, 867-875.
Papanikolaou, S., Chatzifragkou, A., Fakas, S., Galiotou-Panayotou, M., Komaitis, M., Nicaud, J.M. and Aggelis, G. (2009) Biosynthesis of lipids and organic acids by Yarrowia lipolytica strains cultivated on glucose. Eur J Lipid Sci Technol 111, 1221-1232.
Papanikolaou, S., Kampisopoulou, E., Blanchard, F., Rondags, E., Gardeli, Chr., Koutinas, A.A. and Aggelis, G. (2017) Production of secondary metabolites through glycerol fermentation under carbon-excess conditions by the yeasts Yarrowia lipolytica and Rhodosporidium toruloides. Eur J Lipid Sci Technol 119, 1600507.
Patel, A., Pravez, M., Deeba, F., Pruthi, V., Singh, R.P. and Pruthi, P.A. (2014) Boosting accumulation of neutral lipids in Rhodosporidium kratochvilovae HIMPA1 grown on hemp (Cannabis sativa Linn) seed aqueous extract as feedstock for biodiesel production. Bioresour Technol 165, 214-222.
Patel, A., Sindhu, D.K., Arora, N., Singh, R.P., Pruthi, V. and Pruthi, P.A. (2015) Biodiesel production from non-edible lignocellulosic biomass of Cassia fistula L. fruit pulp using oleaginous yeast Rhodosporidium kratochvilovae HIMPA1. Bioresour Technol 197, 91-98.
Patel, A., Arora, N., Km, S., Pruthi, V. and Pruthi, P.A. (2016) Sustainable biodiesel production from oleaginous yeasts utilizing hydrolysates of various non-edible lignocellulosic biomasses. Renew Sustain Energy Rev 62, 836-855.
Patel, A., Arora, N., Pruthi, V. and Pruthi, P.A. (2017) Biological treatment of pulp and paper industry effluent by oleaginous yeast integrated with production of biodiesel as sustainable transportation fuel. J Clean Prod 142, 2858-2864.
Philippoussis, A. and Diamantopoulou, P. (2012) Exploitation of the biotechnological potential of agro-industrial by-products through mushroom cultivation. In Mushroom Biotechnology and Bioengineering ed. Petre, M. and Berovic, M. pp. 161-184. Bucharest: CD Press, University of Pitesti.
Rakicka, M., Kieron, A., Hapeta, P., Neuvéglise, C. and Lazar, Z. (2016a) Sweet and sour potential of yeast from the Yarrowia clade. Biomass Bioenerg 92, 48-54.
Rakicka, M., Rywińska, A., Cybulski, K. and Rymowicz, W. (2016b) Enhanced production of erythritol and mannitol by Yarrowia lipolytica in media containing surfactants. Braz J Microbiol 47, 417-423.
Rakicka, M., Rukowicz, B., Rywińska, A., Lazar, Z. and Rymowicz, W. (2016c) Technology of efficient continuous erythritol production from glycerol. J Clean Prod 139, 905-913.
Rakicka, M., Rywińska, A., Lazar, Z. and Rymowicz, W. (2017) Two-stage continuous culture - technology boosting erythritol production. J Clean Prod 168, 420-427.
Ratledge, C. and Wynn, J.P. (2002) The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. Adv Appl Microbiol 51, 1-51.
Redžepović, S., Orlić, S., Sikora, S., Majdak, A. and Pretorius, I.S. (2002) Identification and characterisation of Saccharomyces cerevisiae and Saccharomyces paradoxus strains isolated from Croatian vineyards. Lett Appl Microbiol 35, 305-310.
Rymowicz, W., Rywińska, A. and Marcinkiewicz, M. (2009) High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica. Biotechnol Lett 31, 377-380.
Rymowicz, W., Fatykhova, A.R., Kamzolova, S.V., Rywinska, A. and Morgunov, I.G. (2010) Citric acid production from glycerol-containing waste of biodiesel industry by Yarrowia lipolytica in batch, repeated batch, and cell recycle regimes. Appl Microbiol Biotechnol 87, 971-979.
Rywińska, A., Juszczyk, P., Wojtatowicz, M., Robak, M., Lazar, Z., Tomaszewska, L. and Rymowicz, W. (2013) Glycerol as a promising substrate for Yarrowia lipolytica biotechnological applications. Biomass Bioenerg 48, 148-166.
Saenge, C., Cheirsilp, B., Suksaroge, T.T. and Bourtoom, T. (2011) Potential use of oleaginous red yeast Rhodotorula glutinis for the bioconversion of crude glycerol from biodiesel plant to lipids and carotenoids. Proc Biochem 46, 210-218.
Saha, B.C., Sakakibara, V. and Cotta, M.A. (2007) Production of D-arabitol by a newly isolated Zygosaccharomyces rouxii. J Ind Microbiol Biotechnol 34, 519-523.
Schoch, C.L., Seifert, K.A., Huhndorf, A., Robert, V., Spouge, J.L., Levesque, C.A. and Chen, W. and Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA 109, 6241-6246.
Selbmann, L., Crognale, S. and Petruccioli, M. (2004) Beta-glucan production by Botryosphaeria rhodina in different bench-top bioreactors. J Appl Microbiol 96, 1074-1081.
Spadaro, D. and Gullino, M.L. (2004) State of the art and future prospects of the biological control of postharvest fruit diseases. Int J Food Microbiol 91, 185-194.
Spier, F., Buffon, J.G. and Burkert, C.A.V. (2015) Bioconversion of raw glycerol generated from the synthesis of biodiesel by different oleaginous yeasts: Lipid content and fatty acid profile of biomass. Indian J. Microbiol 55, 415-422.
Tchakouteu, S., Chatzifragkou, A., Kalantzi, O., Koutinas, A.A., Aggelis, G. and Papanikolaou, S. (2015a) Oleaginous yeast Cryptococcus curvatus exhibits interplay between biosynthesis of intracellular sugars and lipids. Eur J Lipid Sci Technol 117, 657-672.
Tchakouteu, S.S., Kalantzi, O., Gardeli, C., Koutinas, A.A., Aggelis, G. and Papanikolaou, S. (2015b) Lipid production by yeasts growing on biodiesel-derived crude glycerol: strain selection and impact of substrate concentration on the fermentation efficiency. J Appl Microbiol 118, 911-927.
Tomaszewska, L., Rywińska, A. and Gladkowski, W. (2012) Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol. J Ind Microbiol Biotechnol 39, 1333-1343.
Tomaszewska-Hetman, L. and Rywińska, A. (2016) Erythritol biosynthesis from glycerol by Yarrowia lipolytica yeast: effect of osmotic pressure. Chem Pap 70, 272-283.
Torriani, S., Zapparoli, G. and Suzzi, G. (1999) Genetic and phenotypic diversity of Saccharomyces sensu stricto strains isolated from Amarone wine. Antonie Van Leeuwenhoek 75, 207-215.
Tsakraklides, V., Kamineni, A., Consiglio, A.L., MacEwen, K., Friedlander, J., Blitzblau, H.G., Hamilton, M.A., Crabtree, D.V. et al. (2018) High-oleate yeast oil without polyunsaturated fatty acids. Biotechnol Biofuel 11, 131.
Uçkun Kiran, E., Trzcinski, A. and Webb, C. (2013) Microbial oil produced from biodiesel by-products could enhance overall production. Bioresour Technol 129, 650-654.
Uprety, B.K., Dalli, S.S. and Rakshit, S.K. (2017a) Bioconversion of crude glycerol to microbial lipid using a robust oleaginous yeast Rhodosporidium toruloides ATCC 10788 capable of growing in the presence of impurities. Energ Conv Manage 135, 117-128.
Uprety, B.K., Venkatesagowda, B. and Rakshit, S.K. (2017b) Current prospects on production of microbial lipid and other value-added products using crude glycerol obtained from biodiesel industries. BioEnerg Res 10, 1117-1137.
White, T.J., Bruns, T., Lee, S. and Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols, A Guide to Methods and Applications ed. Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J. pp. 315-322. San Diego: Academic Press.
Yamamoto, N., Amemiya, H., Yokomori, Y., Shimizu, K. and Totsuka, A. (1991) Electrophoretic karyotypes of wine yeasts. Am J Enol Vit 4, 358-363.
Yang, X., Jin, G., Gong, Z., Shen, H., Bai, F. and Zhao, Z.K. (2014) Recycling biodiesel-derived glycerol by the oleaginous yeast Rhodosporidium toruloides Y4 through the two-stage lipid production process. Biochem Eng J 91, 86-91.
Yoshikawa, J., Habe, H., Morita, T., Fukuoka, T., Imura, T., Iwabuchi, H., Uemura, S., Tamura, T. et al. (2014) Production of D-arabitol from raw glycerol by Candida quercitrusa. Appl Microbiol Biotechnol 98, 2947-2953.
Τryfinopoulou, P., Tsakalidou, E. and Nychas, G.J.E. (2002) Characterization of Pseudomonas spp. associated with spoilage of gilt-head sea-bream stored under various conditions. Appl Environ Microbiol 68, 65-72.
Τryfinopoulou, P., Tsakalidou, E., Vancanneyt, M., Hoste, B., Swings, J. and Nychas, G.J.E. (2006) Identification of Shewanella spp. strains isolated from Mediterranean fish. J Appl Microbiol 103, 711-721.

Auteurs

R Filippousi (R)

Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece.

D Antoniou (D)

Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece.

P Tryfinopoulou (P)

Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece.

A A Nisiotou (AA)

Hellenic Agricultural Organization "Demeter", Institute of Technology of Agricultural Products, Lycovryssi, Greece.

G-J Nychas (GJ)

Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece.

A A Koutinas (AA)

Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece.

S Papanikolaou (S)

Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece.

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