PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner.
Camptothecin
/ metabolism
Cell Nucleolus
/ metabolism
Cell Nucleus
/ metabolism
DNA Damage
Gossypol
/ metabolism
HeLa Cells
Humans
Hydrogen Peroxide
/ metabolism
Mustard Gas
/ analogs & derivatives
Poly (ADP-Ribose) Polymerase-1
/ genetics
Protein Binding
Protein Transport
Werner Syndrome Helicase
/ metabolism
X-ray Repair Cross Complementing Protein 1
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 07 2019
11 07 2019
Historique:
received:
14
11
2018
accepted:
12
06
2019
entrez:
13
7
2019
pubmed:
13
7
2019
medline:
11
11
2020
Statut:
epublish
Résumé
The prime function of nucleoli is ribogenesis, however, several other, non-canonical functions have recently been identified, including a role in genotoxic stress response. Upon DNA damage, numerous proteins shuttle dynamically between the nucleolus and the nucleoplasm, yet the underlying molecular mechanisms are incompletely understood. Here, we demonstrate that PARP1 and PARylation contribute to genotoxic stress-induced nucleolar-nucleoplasmic shuttling of key genome maintenance factors in HeLa cells. Our work revealed that the RECQ helicase, WRN, translocates from nucleoli to the nucleoplasm upon treatment with the oxidizing agent H
Identifiants
pubmed: 31296950
doi: 10.1038/s41598-019-46358-7
pii: 10.1038/s41598-019-46358-7
pmc: PMC6624289
doi:
Substances chimiques
X-ray Repair Cross Complementing Protein 1
0
XRCC1 protein, human
0
2-chloroethyl ethyl sulfide
693-07-2
Hydrogen Peroxide
BBX060AN9V
PARP1 protein, human
EC 2.4.2.30
Poly (ADP-Ribose) Polymerase-1
EC 2.4.2.30
WRN protein, human
EC 3.6.4.12
Werner Syndrome Helicase
EC 3.6.4.12
Gossypol
KAV15B369O
Mustard Gas
T8KEC9FH9P
Camptothecin
XT3Z54Z28A
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10075Références
Science. 2017 Mar 17;355(6330):1152-1158
pubmed: 28302823
DNA Repair (Amst). 2015 Aug;32:10-16
pubmed: 25963443
Nat Commun. 2017 Aug 30;8(1):328
pubmed: 28855503
J Biochem. 1981 Jun;89(6):1889-901
pubmed: 7287663
Mech Ageing Dev. 2005 Nov;126(11):1146-58
pubmed: 16087220
Bioessays. 2018 May;40(5):e1700233
pubmed: 29603296
Curr Biol. 2017 Dec 4;27(23):R1256-R1258
pubmed: 29207261
Trends Mol Med. 2013 Nov;19(11):643-54
pubmed: 23953479
Curr Opin Cell Biol. 2017 Jun;46:81-86
pubmed: 28431265
Mol Cell. 2012 Mar 30;45(6):706-7
pubmed: 22464439
Mutat Res. 2015 Apr;774:40-8
pubmed: 25801465
Nat Rev Mol Cell Biol. 2007 Jul;8(7):574-85
pubmed: 17519961
DNA Repair (Amst). 2009 Mar 1;8(3):286-97
pubmed: 19144573
Mol Cell. 2010 Oct 22;40(2):216-27
pubmed: 20965417
Nat Commun. 2015 Aug 19;6:8088
pubmed: 26286827
Nucleic Acids Res. 2016 Dec 1;44(21):10386-10405
pubmed: 27694308
Biochemistry. 1984 Jul 31;23(16):3771-7
pubmed: 6206890
Mol Cell Proteomics. 2011 Oct;10(10):M111.009241
pubmed: 21778410
ACS Chem Biol. 2013 Jan 18;8(1):179-88
pubmed: 23082994
Nucleic Acids Res. 2009 Jan;37(Database issue):D181-4
pubmed: 18984612
Acta Naturae. 2017 Apr-Jun;9(2):4-16
pubmed: 28740723
J Biol Chem. 1999 Nov 5;274(45):32122-6
pubmed: 10542247
Cell Rep. 2019 Jan 15;26(3):573-581.e5
pubmed: 30650352
J Cell Sci. 2013 Oct 1;126(Pt 19):4414-23
pubmed: 23868975
Semin Cancer Biol. 2016 Jun;37-38:36-50
pubmed: 26721423
EMBO Rep. 2009 Mar;10(3):231-8
pubmed: 19229283
Chromosoma. 2013 Dec;122(6):487-97
pubmed: 24022641
FEBS J. 2017 Dec;284(23):3977-3985
pubmed: 28500793
Annu Rev Biochem. 2014;83:519-52
pubmed: 24606147
Nucleic Acids Res. 2007;35(22):7665-75
pubmed: 17982172
PLoS Genet. 2012 Jan;8(1):e1002442
pubmed: 22242017
PLoS One. 2012;7(6):e35229
pubmed: 22675465
Nucleic Acids Res. 2013 Mar 1;41(5):3115-29
pubmed: 23355608
Antioxid Redox Signal. 2014 Feb 1;20(4):621-39
pubmed: 23879289
Mol Biosyst. 2017 Feb 28;13(3):443-455
pubmed: 28112326
Oncogene. 2018 May;37(18):2351-2366
pubmed: 29429989
Genes Dev. 2002 Aug 15;16(16):2108-19
pubmed: 12183365
Sci Rep. 2018 Apr 30;8(1):6748
pubmed: 29712969
Nucleic Acids Res. 2017 Mar 17;45(5):2546-2557
pubmed: 27965414
Nat Rev Mol Cell Biol. 2017 Oct;18(10):610-621
pubmed: 28676700
Nucleic Acids Res. 2004 Aug 03;32(13):4003-14
pubmed: 15292449
Cell. 2017 Apr 6;169(2):183
pubmed: 28388401
Nat Commun. 2016 Aug 30;8:16083
pubmed: 28853436
Sci Signal. 2012 Sep 11;5(241):pe38
pubmed: 22969157
Ageing Res Rev. 2015 Sep;23(Pt A):12-28
pubmed: 25555679
Int J Mol Sci. 2017 Jul 01;18(7):
pubmed: 28671574
Biochim Biophys Acta. 2015 Jul;1849(7):821-9
pubmed: 25464032
Aging (Albany NY). 2014 Jan;6(1):70-81
pubmed: 24429382
J Cell Sci. 2005 Jan 1;118(Pt 1):211-22
pubmed: 15615785
Mol Biol Cell. 2003 Oct;14(10):4221-9
pubmed: 12937274
Oncogene. 2002 Apr 11;21(16):2447-54
pubmed: 11971179
Mol Cell. 2012 Mar 30;45(6):790-800
pubmed: 22405650
Angew Chem Int Ed Engl. 2015 Feb 16;54(8):2515-9
pubmed: 25565365
Nat Cell Biol. 2014 Aug;16(8):792-803
pubmed: 25064736
Nucleic Acids Res. 2018 Jan 25;46(2):804-822
pubmed: 29216372
Eur Biophys J. 2014 Nov;43(10-11):509-16
pubmed: 25119658
Nature. 2005 Jan 6;433(7021):77-83
pubmed: 15635413