Pharmacological modulation of melanocortin-4 receptor by melanocortin receptor accessory protein 2 in Nile tilapia.
Adaptor Proteins, Signal Transducing
/ metabolism
Amino Acid Sequence
Animals
Cell Membrane
/ metabolism
Cichlids
/ metabolism
Conserved Sequence
Evolution, Molecular
HEK293 Cells
Humans
Phylogeny
Protein Binding
Receptor, Melanocortin, Type 4
/ chemistry
Signal Transduction
Synteny
/ genetics
Tissue Distribution
Melanocortin 2 receptor accessory protein 2
Melanocortin-4 receptor
Tilapia
Journal
General and comparative endocrinology
ISSN: 1095-6840
Titre abrégé: Gen Comp Endocrinol
Pays: United States
ID NLM: 0370735
Informations de publication
Date de publication:
01 10 2019
01 10 2019
Historique:
received:
10
11
2018
revised:
19
05
2019
accepted:
08
07
2019
pubmed:
13
7
2019
medline:
22
1
2020
entrez:
13
7
2019
Statut:
ppublish
Résumé
The melanocortin-4 receptor (MC4R) acts as a member of G-protein coupled receptors and participate in food intake and energy expenditure. Melanocortin 2 receptor accessory protein 2 (MRAP2) plays a critical role in regulating MC4R signaling in mammals and zebrafish. However, evidence on their interaction in other teleost species remains elusive. Here, we cloned and assessed the evolutionary aspect and pharmacological modulation of MRAP2 on MC4R signaling in Nile tilapia (Oreochromis niloticus). Tissue distribution analysis of tmc4r and tmrap2 confirmed their co-expression in the brain region. tMRAP2 protein could form antiparallel homo-dimer and directly interacted with tMC4R in vitro and presence of tMRAP2 led to the reduction of agonist response and surface expression of tMC4R. Overall, our findings provide a comparative overview on the evolutionary conservation, genomic distribution, tissue-specific expression and pharmacological profile of the MC4R and MRAP2 in another non-mammalian teleost.
Identifiants
pubmed: 31299224
pii: S0016-6480(18)30612-9
doi: 10.1016/j.ygcen.2019.113219
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Receptor, Melanocortin, Type 4
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113219Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.