Phylogeny, sequence-typing and virulence profile of uropathogenic Escherichia coli (UPEC) strains from Pakistan.
Drug Resistance, Multiple, Bacterial
/ genetics
Escherichia coli Infections
/ diagnosis
Female
Humans
Male
Pakistan
/ epidemiology
Phylogeny
Prevalence
Urinary Tract Infections
/ diagnosis
Uropathogenic Escherichia coli
/ classification
Virulence
Virulence Factors
/ classification
beta-Lactamases
/ classification
ESBL
MDR
ST131
UPEC
VF genes
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
12 Jul 2019
12 Jul 2019
Historique:
received:
12
04
2019
accepted:
04
07
2019
entrez:
14
7
2019
pubmed:
14
7
2019
medline:
19
9
2019
Statut:
epublish
Résumé
Escherichia coli lineage ST131 predominates across various spectra of extra-intestinal infections, including urinary tract infection (UTI). The distinctive resistance profile, diverse armamentarium of virulence factors and rapid global dissemination of ST131 E. coli makes it an intriguing pathogen. However, not much is known about the prevalence and genetic attributes of ST131 lineage in Pakistan. We estimated prevalence and genetic attributes of E. coli ST131 isolates causing UTI among 155 randomly selected samples. Samples were analyzed for phylogenetic grouping, O-typing and fumC/fimH typing. Isolates were further tested for the ESBL and virulence factors using PCR. Overall, 59% of the UPEC isolates belonged to the phylogenetic group B2, followed by D = 28%, B1 = 8% and A = 5%. Among 18 different Sequence-types, ST131 was the dominant lineage (n = 71; 46%) out of which 72% of the isolates were assigned to the phylogenetic group B2, while 61% adhered to the serogroup O25b. FumC/fimH typing confirmed 49% of the ST131 as H30 sub-types. In this study, significant numbers of the identified ST131 isolates were MDR and 42% showed ESBL phenotypes, out of which 37% carried bla- Conclusively, this study provides important insight into the genetic and virulence attributes of pandemic MDR ST131 strains involved in UTIs. It also highlights higher prevalence of ST131-O25b-H30 UPEC isolates in patients, which was previously unreported from this part of globe.
Sections du résumé
BACKGROUND
BACKGROUND
Escherichia coli lineage ST131 predominates across various spectra of extra-intestinal infections, including urinary tract infection (UTI). The distinctive resistance profile, diverse armamentarium of virulence factors and rapid global dissemination of ST131 E. coli makes it an intriguing pathogen. However, not much is known about the prevalence and genetic attributes of ST131 lineage in Pakistan.
METHODS
METHODS
We estimated prevalence and genetic attributes of E. coli ST131 isolates causing UTI among 155 randomly selected samples. Samples were analyzed for phylogenetic grouping, O-typing and fumC/fimH typing. Isolates were further tested for the ESBL and virulence factors using PCR.
RESULTS
RESULTS
Overall, 59% of the UPEC isolates belonged to the phylogenetic group B2, followed by D = 28%, B1 = 8% and A = 5%. Among 18 different Sequence-types, ST131 was the dominant lineage (n = 71; 46%) out of which 72% of the isolates were assigned to the phylogenetic group B2, while 61% adhered to the serogroup O25b. FumC/fimH typing confirmed 49% of the ST131 as H30 sub-types. In this study, significant numbers of the identified ST131 isolates were MDR and 42% showed ESBL phenotypes, out of which 37% carried bla-
CONCLUSION
CONCLUSIONS
Conclusively, this study provides important insight into the genetic and virulence attributes of pandemic MDR ST131 strains involved in UTIs. It also highlights higher prevalence of ST131-O25b-H30 UPEC isolates in patients, which was previously unreported from this part of globe.
Identifiants
pubmed: 31299909
doi: 10.1186/s12879-019-4258-y
pii: 10.1186/s12879-019-4258-y
pmc: PMC6626394
doi:
Substances chimiques
Virulence Factors
0
beta-Lactamases
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
620Subventions
Organisme : Higher Education Commision, Pakistan
ID : IRSIP-2016
Références
Appl Environ Microbiol. 2000 Oct;66(10):4555-8
pubmed: 11010916
Infect Dis Clin North Am. 2003 Jun;17(2):227-41
pubmed: 12848468
Int J Antimicrob Agents. 2003 Oct;22 Suppl 2:65-72
pubmed: 14527774
J Infect Dis. 2005 Jan 1;191(1):46-50
pubmed: 15593002
J Clin Microbiol. 2006 Dec;44(12):4589-92
pubmed: 17065267
J Clin Microbiol. 2008 Mar;46(3):1076-80
pubmed: 18199778
J Clin Microbiol. 2008 Aug;46(8):2605-12
pubmed: 18579721
Clin Microbiol Infect. 2009 May;15(5):466-72
pubmed: 19260873
Antimicrob Agents Chemother. 2009 Jul;53(7):2733-9
pubmed: 19398649
J Antimicrob Chemother. 2009 Aug;64(2):274-7
pubmed: 19474064
Int J Antimicrob Agents. 2010 Apr;35(4):316-21
pubmed: 20060273
Clin Infect Dis. 2010 Aug 1;51(3):286-94
pubmed: 20572763
PLoS Pathog. 2010 Nov 11;6(11):e1001187
pubmed: 21085611
Appl Environ Microbiol. 2012 Mar;78(5):1353-60
pubmed: 22226951
Ann Clin Microbiol Antimicrob. 2012 Aug 06;11:23
pubmed: 22867028
J Infect Dis. 2013 Mar 15;207(6):919-28
pubmed: 23288927
BMC Microbiol. 2013 Apr 16;13:84
pubmed: 23586437
J Clin Microbiol. 2013 Sep;51(9):2991-9
pubmed: 23843485
J Microbiol Immunol Infect. 2014 Dec;47(6):455-61
pubmed: 24064288
Antimicrob Agents Chemother. 2013 Dec;57(12):6385-8
pubmed: 24080662
Cell Host Microbe. 2013 Dec 11;14(6):664-74
pubmed: 24331464
J Clin Microbiol. 2014 May;52(5):1358-65
pubmed: 24501035
Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5694-9
pubmed: 24706808
Clin Microbiol Rev. 2014 Jul;27(3):543-74
pubmed: 24982321
Pathog Glob Health. 2015 Feb;109(1):26-9
pubmed: 25605466
J Antimicrob Chemother. 2015 Oct;70(10):2757-62
pubmed: 26183183
Microb Pathog. 2015 Dec;89:188-94
pubmed: 26518125
J Nat Sci Biol Med. 2016 Jan-Jun;7(1):22-6
pubmed: 27003964
Microb Drug Resist. 2017 Mar;23(2):224-229
pubmed: 27214282
mSphere. 2016 Nov 23;1(6):
pubmed: 27904884
Clin Infect Dis. 1993 Sep;17(3):448-56
pubmed: 8105983
Infect Immun. 1998 Sep;66(9):4568-71
pubmed: 9712823
Medscape Womens Health. 1996 Mar;1(3):4
pubmed: 9746621