A thermal ink-jet printing approach for evaluating susceptibility of bacteria to antibiotics.
Antimicrobial resistance
Minimum inhibitory concentration
Thermal ink-jet printing
Y-value
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
24
04
2019
revised:
05
07
2019
accepted:
05
07
2019
pubmed:
14
7
2019
medline:
23
6
2020
entrez:
14
7
2019
Statut:
ppublish
Résumé
An inexpensive method for determining minimum inhibitory concentrations (MIC) using ink-jet printing to deposit drug solutions and bacterial suspensions onto agar was developed. Substrate concentrations were varied using a "Y-value", whereby a series of rectangles with the same width and colour but different heights were printed within a fixed unit area. Prior to MIC determination, the printer cartridges used were calibrated using Fast Green dye. The impact of thermal ink-jet printing on bacterial viability was assessed by colony counting and found not to be deleterious. MIC determinations were conducted by printing varying concentrations of the antibiotics onto agar-coated glass slides then printing a thin even film of a known bacterial density of Lactobacillus acidophilus. Broth microdilution was performed simultaneously to validate the results. Slides and well plates were then incubated anaerobically for 48 h. The MIC values obtained for the antibiotics used were within a permissible range for comparison.
Identifiants
pubmed: 31301322
pii: S0167-7012(19)30218-0
doi: 10.1016/j.mimet.2019.105660
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105660Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.