Pooled library screening with multiplexed Cpf1 library.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
17 07 2019
17 07 2019
Historique:
received:
03
10
2018
accepted:
31
05
2019
entrez:
19
7
2019
pubmed:
19
7
2019
medline:
24
12
2019
Statut:
epublish
Résumé
Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed, high-throughput screening strategy that minimizes library size without sacrificing gene targeting efficiency. We demonstrated that AsCpf1 can be used for functional genomics screenings and that an AsCpf1-based multiplexed library performs similarly as compared to currently available monocistronic CRISPR/Cas9 libraries, with only one vector required for each gene. We construct the smallest whole-genome CRISPR knock-out library, Mini-human, for the human genome (n = 17,032 constructs targeting 16,977 protein-coding genes), which performs favorably compared to conventional Cas9 libraries.
Identifiants
pubmed: 31316073
doi: 10.1038/s41467-019-10963-x
pii: 10.1038/s41467-019-10963-x
pmc: PMC6637147
doi:
Substances chimiques
RNA, Guide
0
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3144Subventions
Organisme : NCI NIH HHS
ID : P01 CA117969
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA016672
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130119
Pays : United States
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