Expression, Purification, and Spectral Characterization of Phytochromes.
Difference spectrum
Phytochromes
Pichia pastoris
Recombinant protein
Streptavidin affinity chromatography
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
19
7
2019
pubmed:
19
7
2019
medline:
31
3
2020
Statut:
ppublish
Résumé
Expression and purification of recombinant proteins are important for the structure-function study of phytochromes. However, it is difficult to purify phytochrome proteins from natural sources or using a bacterial expression system, due to the presence of multiple phytochrome species and low expression and solubility, respectively. Here we describe the expression of recombinant full-length plant phytochromes in the yeast Pichia pastoris, and the spectral analysis of chromophore-assembled phytochromes before and after the purification by streptavidin affinity chromatography.
Identifiants
pubmed: 31317405
doi: 10.1007/978-1-4939-9612-4_7
doi:
Substances chimiques
Recombinant Proteins
0
Phytochrome
11121-56-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
95-111Références
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