Phytochrome Regulation of Seed Germination.
Light
Phytochrome
Seed dormancy
Seed germination
phyA
phyB
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
19
7
2019
pubmed:
19
7
2019
medline:
31
3
2020
Statut:
ppublish
Résumé
Seed germination assays consist of counting the number of germinated seeds, defined as seeds in which the radicle has ruptured the endosperm and emerged from the seed coat. In Arabidopsis seed germination assays, Arabidopsis seeds are surface-sterilized, plated on agar plates containing test compounds, and incubated at specific temperatures under specific light conditions, after which the germinated seeds are counted, either with the naked eye or under a microscope. This chapter describes step-by-step protocols for Arabidopsis seed germination assays under phytochrome-dependent conditions.
Identifiants
pubmed: 31317410
doi: 10.1007/978-1-4939-9612-4_12
doi:
Substances chimiques
Arabidopsis Proteins
0
Phytochrome A
0
Phytochrome
11121-56-5
Phytochrome B
136250-22-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
149-156Références
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