Genetic transformation of the dinoflagellate chloroplast.
Amphidinium
chloroplast
coral reef
dinoflagellate
ecology
transformation
zooxanthella
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
18 07 2019
18 07 2019
Historique:
received:
17
01
2019
accepted:
06
07
2019
entrez:
19
7
2019
pubmed:
19
7
2019
medline:
18
12
2019
Statut:
epublish
Résumé
Coral reefs are some of the most important and ecologically diverse marine environments. At the base of the reef ecosystem are dinoflagellate algae, which live symbiotically within coral cells. Efforts to understand the relationship between alga and coral have been greatly hampered by the lack of an appropriate dinoflagellate genetic transformation technology. By making use of the plasmid-like fragmented chloroplast genome, we have introduced novel genetic material into the dinoflagellate chloroplast genome. We have shown that the introduced genes are expressed and confer the expected phenotypes. Genetically modified cultures have been grown for 1 year with subculturing, maintaining the introduced genes and phenotypes. This indicates that cells continue to divide after transformation and that the transformation is stable. This is the first report of stable chloroplast transformation in dinoflagellate algae.
Identifiants
pubmed: 31317866
doi: 10.7554/eLife.45292
pii: 45292
pmc: PMC6639071
doi:
pii:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L014130/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_14116
Pays : United Kingdom
Organisme : Gordon and Betty Moore Foundation
ID : GBMF4976.01
Pays : International
Organisme : King Abdullah University of Science and Technology
ID : URF/1/2216/01-01
Pays : International
Informations de copyright
© 2019, Nimmo et al.
Déclaration de conflit d'intérêts
IN, AB, IL, JC, KG, AS, MA, SP, RW, RN, CH No competing interests declared
Références
Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8007-12
pubmed: 10393938
Nature. 1999 Jul 8;400(6740):155-9
pubmed: 10408440
Mol Gen Genet. 2000 Feb;263(1):152-8
pubmed: 10732684
Mol Genet Genomics. 2001 Dec;266(4):632-8
pubmed: 11810235
Mol Biol Evol. 2002 Apr;19(4):489-500
pubmed: 11919290
Trends Genet. 2004 May;20(5):261-7
pubmed: 15109781
Protist. 2007 Jan;158(1):89-103
pubmed: 17049921
BMC Genomics. 2006 Nov 23;7:297
pubmed: 17123435
Science. 1983 Dec 23;222(4630):1346-9
pubmed: 17773338
J Exp Bot. 2008;59(5):1035-45
pubmed: 18319241
Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot5439
pubmed: 20516177
Plant Mol Biol. 2012 Jul;79(4-5):347-57
pubmed: 22562591
Protist. 2014 Jan;165(1):1-13
pubmed: 24316380
PLoS One. 2015 Jul 13;10(7):e0132693
pubmed: 26167858
Science. 2015 Nov 13;350(6262):769-71
pubmed: 26564846
Biochim Biophys Acta. 2016 Jun;1857(6):840-7
pubmed: 26869375
New Phytol. 2016 Oct;212(2):472-84
pubmed: 27321415
Front Plant Sci. 2017 Feb 28;8:271
pubmed: 28293249
Nature. 2017 Mar 15;543(7645):373-377
pubmed: 28300113
Science. 1988 Jun 10;240(4858):1534-8
pubmed: 2897716
Brief Funct Genomics. 2018 Jan 1;17(1):26-33
pubmed: 29365068
PLoS One. 2019 Feb 19;14(2):e0211936
pubmed: 30779749
Nucleic Acids Res. 1984 Dec 21;12(24):9489-96
pubmed: 6514581