Sequencing and analysis of globally obtained human parainfluenza viruses 1 and 3 genomes.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2019
Historique:
received: 25 01 2019
accepted: 08 07 2019
entrez: 19 7 2019
pubmed: 19 7 2019
medline: 4 3 2020
Statut: epublish

Résumé

Human Parainfluenza viruses (HPIV) type 1 and 3 are important causes of respiratory tract infections in young children globally. HPIV infections do not confer complete protective immunity so reinfections occur throughout life. Since no effective vaccine is available for the two virus subtypes, comprehensive understanding of HPIV-1 and HPIV-3 genetic and epidemic features is important for diagnosis, prevention, and treatment of HPIV-1 and HPIV-3 infections. Relatively few whole genome sequences are available for both HPIV-1 and HPIV-3 viruses, so our study sought to provide whole genome sequences from multiple countries to further the understanding of the global diversity of HPIV at a whole-genome level. We collected HPIV-1 and HPIV-3 samples and isolates from Argentina, Australia, France, Mexico, South Africa, Switzerland, and USA from the years 2003-2011 and sequenced the genomes of 40 HPIV-1 and 75 HPIV-3 viruses with Sanger and next-generation sequencing with the Ion Torrent, Illumina, and 454 platforms. Phylogenetic analysis showed that the HPIV-1 genome is evolving at an estimated rate of 4.97 × 10-4 mutations/site/year (95% highest posterior density 4.55 × 10-4 to 5.38 × 10-4) and the HPIV-3 genome is evolving at a similar rate (3.59 × 10-4 mutations/site/year, 95% highest posterior density 3.26 × 10-4 to 3.94 × 10-4). There were multiple genetically distinct lineages of both HPIV-1 and 3 circulating on a global scale. Further surveillance and whole-genome sequencing are greatly needed to better understand the spatial dynamics of these important respiratory viruses in humans.

Identifiants

pubmed: 31318956
doi: 10.1371/journal.pone.0220057
pii: PONE-D-19-02466
pmc: PMC6638977
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0220057

Subventions

Organisme : NIAID NIH HHS
ID : HHSN272200900007C
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI110819
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI070428
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI077988
Pays : United States

Déclaration de conflit d'intérêts

I have read the journal's policy and the authors of this manuscript have the following competing interests: Daniel E. Noyola has participated as a member of the speakers' bureau of AbbVie and speakers' bureau and advisory board for Sanofi Pasteur. This does not alter our adherence to PLOS ONE policies on sharing data and materials.

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Auteurs

Michael E Bose (ME)

Midwest Respiratory Virus Program, Medical College of Wisconsin, Milwaukee, WI, United States of America.

Susmita Shrivastava (S)

J. Craig Venter Institute, Rockville, MD, United States of America.

Jie He (J)

Midwest Respiratory Virus Program, Medical College of Wisconsin, Milwaukee, WI, United States of America.

Martha I Nelson (MI)

Fogarty International Center, National Institutes of Health, Bethesda, MD, United States of America.

Jayati Bera (J)

J. Craig Venter Institute, Rockville, MD, United States of America.

Nadia Fedorova (N)

J. Craig Venter Institute, Rockville, MD, United States of America.

Rebecca Halpin (R)

J. Craig Venter Institute, Rockville, MD, United States of America.

Christopher D Town (CD)

J. Craig Venter Institute, Rockville, MD, United States of America.

Hernan A Lorenzi (HA)

J. Craig Venter Institute, Rockville, MD, United States of America.

Paolo Amedeo (P)

J. Craig Venter Institute, Rockville, MD, United States of America.

Neha Gupta (N)

J. Craig Venter Institute, Rockville, MD, United States of America.

Daniel E Noyola (DE)

Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.

Cristina Videla (C)

Clinical Virology Laboratory, Centro de Educación Médica e Investigaciones Clínicas (CEMIC) University Hospital, Buenos Aires, Argentina.

Tuckweng Kok (T)

School of Molecular and Biomedical Science, University of Adelaide, Adelaide, Australia.

Amelia Buys (A)

Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases, Sandringham, South Africa.

Marietjie Venter (M)

Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases, Sandringham, South Africa.
Zoonotic, arbo and respiratory virus program, Department Medical Virology, University of Pretoria, Pretoria, South Africa.

Astrid Vabret (A)

Normandie Université, Caen, France.
Groupe de Recherche sur l'Adaptation Microbienne (GRAM), Université de Caen, Caen, France.
Laboratoire de Virologie, Centre Hospitalier Universitaire de Caen, Caen, France.

Samuel Cordey (S)

Division of Infectious Diseases and Laboratory of Virology, University of Geneva Hospitals, Geneva, Switzerland.

Kelly J Henrickson (KJ)

Midwest Respiratory Virus Program, Medical College of Wisconsin, Milwaukee, WI, United States of America.

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Classifications MeSH