Economical production of androstenedione and 9α-hydroxyandrostenedione using untreated cane molasses by recombinant mycobacteria.

Androstenedione Canes Molasses Mycobacterium Nitrogen
Cane molasses Hydrolysate of mycobacteria cells Mycobacteria Phytosterol biotransformation Propionyl-CoA carboxylase

Journal

Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523

Informations de publication

Date de publication:
Oct 2019
Historique:
received: 09 05 2019
revised: 29 06 2019
accepted: 01 07 2019
pubmed: 22 7 2019
medline: 19 9 2019
entrez: 21 7 2019
Statut: ppublish

Résumé

Production of androstenedione (AD) and 9α-hydroxyandrostenedione (9α-OH-AD) by recombinant mycobacteria using untreated cane molasses and hydrolysate of mycobacterial cells (HMC) was investigated for the first time. B-vitamins feeding experiment and reverse transcription-PCR analysis showed that propionyl-CoA carboxylase (PCC) plays an important role in the phytosterol biotransformation of mycobacteria. The respective AD and 9α-OH-AD conversion ratios were increased by 2.91 and 1.48 times through coexpression of PCC and NADH dehydrogenase. The highest conversion ratios of AD and 9α-OH-AD obtained by using a co-feeding strategy of cane molasses and HMC reached 96.38% and 95.04%, respectively, and the total costs of carbon and nitrogen sources for the culture medium were reduced by 29.89% and 49.49%, respectively. Taking the results together, untreated cane molasses and HMC can be used for the economical production of steroidal pharmaceutical precursors by mycobacteria. This study offers an economical and green strategy for steroidal pharmaceutical precursor production.

Identifiants

DOI: 10.1016/j.biortech.2019.121750 PMID: 31325842
pubmed: 31325842
pii: S0960-8524(19)30980-0
doi: 10.1016/j.biortech.2019.121750
pii:
doi:

Substances chimiques

Androstenedione 409J2J96VR
Nitrogen N762921K75

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121750

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Xiuling Zhou (X)

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.

Yang Zhang (Y)

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; College of Life Science, Liaocheng University, Liaocheng, Shandong 252059, China. Electronic address: zhy@lcu.edu.cn.

Yanbing Shen (Y)

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, 300457 Tianjin, China.

Xiao Zhang (X)

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.

Zhenjian Zhang (Z)

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.

Shuangping Xu (S)

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.

Jianmei Luo (J)

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.

Menglei Xia (M)

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.

Min Wang (M)

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, 300457 Tianjin, China. Electronic address: minw@tust.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Hormones gonadiques › Hormones sexuelles stéroïdiennes › Congénères de la testostérone › Androstènedione › Economical production of androstenedione and...
questionsmedicales.fr › Équipement et fournitures › Équipement chirurgical › Équipement orthopédique › Cannes › Economical production of androstenedione and...
questionsmedicales.fr › Aliments et boissons › Aliments › Mélasses › Economical production of androstenedione and...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Mycobacteriaceae › Mycobacterium › Economical production of androstenedione and...
questionsmedicales.fr › Produits chimiques inorganiques › Gaz › Azote › Economical production of androstenedione and...