TLR9 agonist adsorbed to alum adjuvant prevents asthma-like responses induced by Blomia tropicalis mite extract.
Adjuvants, Immunologic
/ pharmacology
Adsorption
Alum Compounds
/ chemistry
Anaphylaxis
/ complications
Animals
Asthma
/ complications
Cytokines
/ biosynthesis
Disease Models, Animal
Eosinophils
/ pathology
Female
Hypersensitivity
/ complications
Immunity
/ drug effects
Immunization
Interleukin-10
/ metabolism
Interleukin-4
/ biosynthesis
Lymph Nodes
/ drug effects
Lymphocyte Subsets
/ drug effects
Mice, Inbred C57BL
Neutrophils
/ pathology
Oligodeoxyribonucleotides
/ pharmacology
Pyroglyphidae
/ drug effects
Th1 Cells
/ drug effects
Th2 Cells
/ drug effects
Toll-Like Receptor 9
/ agonists
Blomia tropicalis
CpG-ODN
bronchoalveolar lavage
inflammation
lungs
Journal
Journal of leukocyte biology
ISSN: 1938-3673
Titre abrégé: J Leukoc Biol
Pays: England
ID NLM: 8405628
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
04
01
2019
revised:
24
06
2019
accepted:
27
06
2019
pubmed:
23
7
2019
medline:
27
5
2020
entrez:
23
7
2019
Statut:
ppublish
Résumé
Blomia tropicalis mite is highly prevalent in tropical and subtropical regions and it is associated with allergic diseases such as rhinitis and asthma. By using an OVA-model of allergic lung disease, we have previously shown that sensitization in the presence of toll like receptors (TLRs) agonists attenuates subsequent OVA-induced allergic responses. Here, we evaluated the effect of CpG-ODN, a specific synthetic TLR-9 agonist, on the development of experimental asthma induced by Blomia tropicalis extract, a relevant source of aeroallergens. Among different protocols of Blomia tropicalis extract sensitization, the subcutaneous sensitization in the presence of alum adjuvant induced the highest Th2 responses, including high IgE levels. Adsorption of CpG to Blomia tropicalis extract/Alum attenuated the airway hyperreactivity, the infiltration of inflammatory cells including eosinophils, and the IL-5 content in BAL. In addition, lung peribronchial inflammatory infiltrate, mucus production and IL-5-producing CD3
Identifiants
pubmed: 31329326
doi: 10.1002/JLB.MA1218-475RR
doi:
Substances chimiques
Adjuvants, Immunologic
0
Alum Compounds
0
CPG-oligonucleotide
0
Cytokines
0
Oligodeoxyribonucleotides
0
Toll-Like Receptor 9
0
Interleukin-10
130068-27-8
Interleukin-4
207137-56-2
aluminum sulfate
34S289N54E
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
653-664Informations de copyright
©2019 Society for Leukocyte Biology.
Références
Global Asthma Network. The Global Asthma Report 2014. Auckland, New Zeal. 2014. ISBN: 978-0-473-29125-9. http://www.globalasthmanetwork.org/publications/Global_Asthma_Report_2014.pdf
BRASIL. Doenças Respiratórias Crônicas. Ministério da Saúde 2010. ISBN: 978-85-334-1699-4. https://bvsms.saude.gov.br/bvs/publicacoes/doencas_respiratorias_cronicas.pdf
Barnes PJ. Molecular mechanisms of atopy. Mediat Inflamm. 2001;10:285-288.
Barnes PJ. Asthma mechanisms. Medicine 2016;42:265-270.
Sade K, Roitman D, Kivity S. Sensitization to dermatophagoides, Blomia tropicalis, and other mites in atopic patients. J Asthma. 2010;47:849-852.
Chua KY, Cheong N, Kuo IC, et al. The Blomia tropicalis allergens. Protein Pept Lett. 2007;14:325-333.
Pereira EA, Silva DA, Cunha-Júnior JP, et al. IgE, IgG1, and IgG4 antibody responses to Blomia tropicalis in atopic patients. Allergy Eur J Allergy Clin Immunol. 2005;60:401-406.
Dullaers M, Schuijs MJ, Willart M, et al. House dust mite-driven asthma and allergen-specific T cells depend on B cells when the amount of inhaled allergen is limiting. J Allergy Clin Immunol. 2017;140:76-88.e7.
Barboza R, Câmara NO, Gomes E, et al. Endotoxin exposure during sensitization to Blomia tropicalis allergens shifts TH2 immunity towards a TH17-mediated airway neutrophilic inflammation: role of TLR4 and TLR2. PLoS One 2013;8:e67115.
Bortolatto J, Borducchi E, Rodriguez D, et al. Toll-like receptor 4 agonists adsorbed to aluminium hydroxide adjuvant attenuate ovalbumin-specific allergic airway disease: role of MyD88 adaptor molecule and interleukin-12/interferon-γ axis. Clin Exp Allergy. 2008;38:1668-1679.
Mirotti L, Alberca Custódio RW, Gomes E, et al. CpG-ODN shapes alum adjuvant activity signaling via MyD88 and IL-10. Front Immunol. 2017;8:74.
Steinhagen F, Kinjo T, Bode C, Klinman DM. TLR-based immune adjuvants. Vaccine 2011;29:3341-3355.
Faustino L, Mucida D, Keller AC, et al. Regulatory T cells accumulate in the lung allergic inflammation and efficiently suppress T-cell proliferation but not Th2 cytokine production. Clin Dev Immunol. 2012;2012:721817.
Evans H, Killoran KE, Mitre E. Measuring local anaphylaxis in mice. J Vis Exp. 2014;92:e52005.
Makabe-Kobayashi Y, Hori Y, Adachi T, et al. The control effect of histamine on body temperature and respiratory function in IgE-dependent systemic anaphylaxis. J Allergy Clin Immunol. 2002;110:298-303.
Zakeri A, Russo M. Dual role of toll-like receptors in human and experimental asthma models. Front Immunol. 2018;9:1027.
Besnard AG, Guillou N, Tschopp J, et al. NLRP3 inflammasome is required in murine asthma in the absence of aluminum adjuvant. Allergy Eur J Allergy Clin Immunol. 2011;66:1047-1057.
De Nardo D, De Nardo CM, Latz E. New insights into mechanisms controlling the NLRP3 inflammasome and its role in lung disease. Am J Pathol. 2014;184:42-54.
Thomas SY, Whitehead GS, Takaku M et al. MyD88-dependent dendritic and epithelial cell crosstalk orchestrates immune responses to allergens. Mucosal Immunol. 2018;11:796-810.
Eisenbarth SC. Use and limitations of alum-based models of allergy. Clin Exp Allergy. 2008;38:1572-1575.
León B, Ballesteros-Tato A, Lund FE. Dendritic cells and B cells: unexpected partners in Th2 development. J Immunol. 2014;193:1531-1537.
Heeg K, Zimmermann S. CpG DNA as a Th1 trigger. Int Arch Allergy Immunol. 2000;121:87-97.
Urry Z, Xystrakis E, Richards DF, et al. Ligation of TLR9 induced on human IL-10-secreting Tregs by 1α,25-dihydroxyvitamin D3 abrogates regulatory function. J Clin Invest. 2009;119:387-398.
Kim DH, Sohn J-H, Park H-J, Lee J-H, Park J-W, Choi J-M. CpG oligodeoxynucleotide inhibits cockroach-induced asthma via induction of ifn-γ+th1 cells or foxp3+regulatory t cells in the lung. Allergy, Asthma Immunol Res. 2016;8:264.
Vicari AP, Chiodoni C, Vaure C, et al. Reversal of tumor-induced dendritic cell paralysis by CpG immunostimulatory oligonucleotide and anti-interleukin 10 receptor antibody. J Exp Med. 2002;196:541.
Bortolatto J, Mirotti L, Rodriguez D, Gomes E, Russo M. Adsorption of Toll-like receptor 4 agonist to alum-based tetanus toxoid vaccine dampens pro-T helper 2 activities and enhances antibody responses. J Immunol Res. 2015;2015:280238.
Creticos PS, Schroeder JT, Hamilton RG, et al. Immune tolerance network, immunotherapy with a ragweed-toll-like receptor 9 agonist vaccine for allergic rhinitis. N Engl J Med. 2006. https://doi.org/10.1056/NEJMoa052916.
Beeh KM, Kanniess F, Wagner F, et al. The novel TLR-9 agonist QbG10 shows clinical efficacy in persistent allergic asthma. J Allergy Clin Immunol. 2013. https://doi.org/10.1016/j.jaci.2012.12.1561.