Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovative Bioconjugation.

Chitin / chemistry Click Chemistry / methods Inteins / genetics Protein Binding / genetics Protein Domains / genetics Protein Engineering / methods Single-Domain Antibodies / chemistry
Click chemistry Intein Nanobody Protein functionalization

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 24 7 2019
pubmed: 25 7 2019
medline: 9 4 2020
Statut: ppublish

Résumé

An expression strategy is presented in order to produce nanobodies modified with a clickable alkyne functionality at their C-terminus via the intein-mediated protein ligation (IPL) technique. The protocol focuses on the cytoplasmic expression and extraction of a nanobody-intein-chitin binding domain (CBD) fusion protein in E. coli SHuffle

Identifiants

DOI: 10.1007/978-1-4939-9654-4_9 PMID: 31332751
pubmed: 31332751
doi: 10.1007/978-1-4939-9654-4_9
doi:

Substances chimiques

Single-Domain Antibodies 0
Chitin 1398-61-4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

117-130

Auteurs

Geert-Jan Graulus (GJ)

Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, Diepenbeek, Belgium.

Duy Tien Ta (DT)

Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, Diepenbeek, Belgium.
Downstream Processing Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A∗STAR), Singapore, Singapore.

Huong Tran (H)

Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, Diepenbeek, Belgium.

Rebekka Hansen (R)

Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, Diepenbeek, Belgium.

Brecht Billen (B)

Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, Diepenbeek, Belgium.

Erik Royackers (E)

Biomedical Research Institute (Biomed), Hasselt University, Diepenbeek, Belgium.

Jean-Paul Noben (JP)

Biomedical Research Institute (Biomed), Hasselt University, Diepenbeek, Belgium.

Nick Devoogdt (N)

In Vivo Cellular and Molecular Imaging Laboratory (ICMI), Vrije Universiteit Brussel, Brussels, Belgium.

Serge Muyldermans (S)

Cellular and Molecular Immunology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium.

Wanda Guedens (W)

Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, Diepenbeek, Belgium.

Peter Adriaensens (P)

Biomolecule Design Group, Institute for Materials Research (IMO), Hasselt University, Diepenbeek, Belgium. peter.adriaensens@uhasselt.be.
Applied and Analytical Chemistry, Institute for Materials Research (IMO), Hasselt University, Diepenbeek, Belgium. peter.adriaensens@uhasselt.be.

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Classifications MeSH

questionsmedicales.fr › Glucides › Polyosides › Chitine › Site-Selective Functionalization of Nanobodies...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Chimie click › Site-Selective Functionalization of Nanobodies...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Intéines › Site-Selective Functionalization of Nanobodies...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Site-Selective Functionalization of Nanobodies...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Site-Selective Functionalization of Nanobodies...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Site-Selective Functionalization of Nanobodies...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Fragments d'immunoglobuline › Fragments Fab d'immunoglobuline › Anticorps à domaine unique › Site-Selective Functionalization of Nanobodies...