CD93 is a cell surface lectin receptor involved in the control of the inflammatory response stimulated by exogenous DNA.
Antigens, CD
/ genetics
Biological Transport
/ genetics
Cell Line, Tumor
Cloning, Molecular
DNA, Bacterial
/ genetics
Endosomes
/ immunology
Escherichia coli
/ chemistry
Gene Expression
Gene Expression Regulation
/ immunology
Genetic Vectors
/ chemistry
Humans
Inflammation
Interleukin-6
/ genetics
Lectins, C-Type
/ genetics
Membrane Glycoproteins
/ genetics
Minor Histocompatibility Antigens
/ genetics
Models, Biological
Neurons
/ immunology
Oligodeoxyribonucleotides
/ genetics
Protein Binding
Protein Domains
Receptors, Cell Surface
/ genetics
Receptors, Complement
/ genetics
Recombinant Fusion Proteins
/ genetics
Signal Transduction
Toll-Like Receptor 9
/ genetics
C-type lectin-like domain
CD93
CpG oligonucleotide
Toll-like receptor 9
bacterial DNA
inflammation
Journal
Immunology
ISSN: 1365-2567
Titre abrégé: Immunology
Pays: England
ID NLM: 0374672
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
09
11
2018
revised:
11
07
2019
accepted:
11
07
2019
pubmed:
25
7
2019
medline:
9
1
2020
entrez:
24
7
2019
Statut:
ppublish
Résumé
Bacterial DNA contains CpG oligonucleotide (ODN) motifs to trigger innate immune responses through the endosomal receptor Toll-like receptor 9 (TLR9). One of the cell surface receptors to capture and deliver microbial DNA to intracellular TLR9 is the C-type lectin molecule DEC-205 through its N-terminal C-type lectin-like domain (CTLD). CD93 is a cell surface protein and member of the lectin group XIV with a CTLD. We hypothesized that CD93 could interact with CpG motifs, and possibly serve as a novel receptor to deliver bacterial DNA to endosomal TLR9. Using ELISA and tryptophan fluorescence binding studies we observed that the soluble histidine-tagged CD93-CTLD was specifically binding to CpG ODN and bacterial DNA. Moreover, we found that CpG ODN could bind to CD93-expressing IMR32 neuroblastoma cells and induced more robust interleukin-6 secretion when compared with mock-transfected IMR32 control cells. Our data argue for a possible contribution of CD93 to control cell responsiveness to bacterial DNA in a manner reminiscent of DEC-205. We postulate that CD93 may act as a receptor at plasma membrane for DNA or CpG ODN and to grant delivery to endosomal TLR9.
Identifiants
pubmed: 31335975
doi: 10.1111/imm.13100
pmc: PMC6742780
doi:
Substances chimiques
Antigens, CD
0
CPG-oligonucleotide
0
DEC-205 receptor
0
DNA, Bacterial
0
IL6 protein, human
0
Interleukin-6
0
Lectins, C-Type
0
Membrane Glycoproteins
0
Minor Histocompatibility Antigens
0
Oligodeoxyribonucleotides
0
Receptors, Cell Surface
0
Receptors, Complement
0
Recombinant Fusion Proteins
0
TLR9 protein, human
0
Toll-Like Receptor 9
0
complement 1q receptor
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
85-93Informations de copyright
© 2019 John Wiley & Sons Ltd.
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