Ssy1 functions at the plasma membrane as a receptor of extracellular amino acids independent of plasma membrane-endoplasmic reticulum junctions.
Amino Acids
/ metabolism
Cell Membrane
/ metabolism
Endoplasmic Reticulum
/ metabolism
Intracellular Signaling Peptides and Proteins
/ chemistry
Membrane Proteins
/ chemistry
Protein Binding
Protein Sorting Signals
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
/ chemistry
Signal Transduction
ER-PM tethering
Saccharomyces cerevisiae
amino acid permease
amino acid signaling
endoplasmic reticulum
membrane protein trafficking
plasma membrane
Journal
Traffic (Copenhagen, Denmark)
ISSN: 1600-0854
Titre abrégé: Traffic
Pays: England
ID NLM: 100939340
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
12
04
2019
revised:
17
07
2019
accepted:
18
07
2019
pubmed:
25
7
2019
medline:
16
5
2020
entrez:
24
7
2019
Statut:
ppublish
Résumé
Evidence from multiple laboratories has implicated Ssy1, a nontransporting amino acid permease, as the receptor component of the yeast plasma membrane (PM)-localized SPS (Ssy1-Ptr3-Ssy5)-sensor. Upon binding external amino acids, Ssy1 is thought to initiate signaling events leading to the induction of amino acid permease gene expression. In striking contrast, Kralt et al (2015) (Traffic 16:135-147) have questioned the role of Ssy1 in amino acid sensing and reported that Ssy1 is a component of the endoplasmic reticulum (ER), where it reportedly participates in the formation of ER-PM junctions. Here, we have re-examined the intracellular location of Ssy1 and tested the role of ER-PM junctions in SPS sensor signaling. We show that the C-terminal of Ssy1 carries a functional ER-export motif required for proper localization of Ssy1 to the PM. Furthermore, ER-PM junctions are dispensable for PM-localization and function of Ssy1; Ssy1 localizes to the PM in a Δtether strain lacking ER-PM junctions (ist2Δ scs2Δ scs22Δ tcb1Δ tcb2Δ tcb3Δ), and this strain retains the ability to initiate signals induced by extracellular amino acids. The data demonstrate that Ssy1 functions as the primary amino acid receptor and that it carries out this function at the PM.
Substances chimiques
Amino Acids
0
Intracellular Signaling Peptides and Proteins
0
Membrane Proteins
0
Protein Sorting Signals
0
SSY1 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
775-784Informations de copyright
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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