Dose-dependent metabolite changes after ethanol intoxication in rat prefrontal cortex using in vivo magnetic resonance spectroscopy.
Alcoholic Intoxication
/ metabolism
Animals
Choline
/ metabolism
Creatine
/ metabolism
Dose-Response Relationship, Drug
Ethanol
/ toxicity
Glutamic Acid
/ metabolism
Glutamine
/ metabolism
Magnetic Resonance Spectroscopy
Male
Prefrontal Cortex
/ drug effects
Rats
Rats, Wistar
gamma-Aminobutyric Acid
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 07 2019
23 07 2019
Historique:
received:
21
08
2018
accepted:
10
07
2019
entrez:
25
7
2019
pubmed:
25
7
2019
medline:
24
10
2020
Statut:
epublish
Résumé
Ethanol disrupts the balance between the excitatory (glutamatergic) and inhibitory (GABAergic) neurotransmission systems. We aimed to assess how acute ethanol intoxication in rats affects the levels of GABA, glutamate and other cerebral metabolites after injection of two different doses of ethanol. One in vivo magnetic resonance spectrum of the prefrontal cortex region was acquired before and six spectra were acquired after intraperitoneal injections of saline or ethanol (1 g/kg or 2 g/kg). Brain kinetics after exposure to ethanol were compared to blood ethanol kinetics. GABA levels significantly decreased after injection of 1 g/kg but not 2 g/kg doses of ethanol. Choline levels, which serve as a marker of alterations in membrane composition, significantly decreased after injection of 2 g/kg but not 1 g/kg doses of ethanol. Acute ethanol intoxication appears to result in specific dose-dependent changes in the GABA level and choline level.
Identifiants
pubmed: 31337845
doi: 10.1038/s41598-019-47187-4
pii: 10.1038/s41598-019-47187-4
pmc: PMC6650461
doi:
Substances chimiques
Glutamine
0RH81L854J
Ethanol
3K9958V90M
Glutamic Acid
3KX376GY7L
gamma-Aminobutyric Acid
56-12-2
Creatine
MU72812GK0
Choline
N91BDP6H0X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10682Références
Neuroscience. 2014 Mar 14;262:107-17
pubmed: 24406441
Neuropsychopharmacology. 2013 May;38(6):1121-9
pubmed: 23306181
J Neurochem. 2014 Mar;128(5):628-40
pubmed: 24164397
Int J Mol Sci. 2017 Aug 21;18(8):
pubmed: 28825683
Neuropsychopharmacology. 2006 Dec;31(12):2683-91
pubmed: 16407891
AJNR Am J Neuroradiol. 2013 Feb;34(2):259-65
pubmed: 22268095
J Cereb Blood Flow Metab. 2009 May;29(5):891-902
pubmed: 19240741
Neuroscience. 2008 Apr 22;153(1):259-67
pubmed: 18358631
Addict Biol. 2016 Nov;21(6):1199-1216
pubmed: 26283309
Biol Psychiatry. 2010 May 1;67(9):846-54
pubmed: 20044076
Neuroimage. 2014 Feb 1;86:43-52
pubmed: 23246994
MAGMA. 2001 May;12(2-3):141-52
pubmed: 11390270
NMR Biomed. 2000 May;13(3):129-53
pubmed: 10861994
J Chromatogr. 1987 Feb 20;414(1):248-50
pubmed: 3571391
Nat Rev Neurosci. 2011 Jun 02;12(7):400-13
pubmed: 21633380
J Physiol Paris. 2013 Dec;107(6):471-82
pubmed: 23684970
Alcohol Clin Exp Res. 2014 Apr;38(4):969-79
pubmed: 24512596
Eur J Pharmacol. 1985 May 20;111(3):365-8
pubmed: 2990958
Prog Nucl Magn Reson Spectrosc. 2012 Jan;60:29-41
pubmed: 22293397
Biomed Res Int. 2014;2014:351903
pubmed: 24783201
J Magn Reson. 1997 Nov;129(1):35-43
pubmed: 9405214
Magn Reson Med. 1999 Apr;41(4):649-56
pubmed: 10332839
J Chromatogr B Biomed Sci Appl. 1997 Dec 19;704(1-2):365-8
pubmed: 9518172
Biol Psychiatry. 2012 Feb 1;71(3):239-46
pubmed: 21855054