Diverse glutamatergic inputs target spines expressing M1 muscarinic receptors in the basolateral amygdala: An ultrastructural analysis.
Amygdala
Electron microscopy
Immunohistochemistry
M1 muscarinic receptors
VGluT1
VGluT2
Journal
Brain research
ISSN: 1872-6240
Titre abrégé: Brain Res
Pays: Netherlands
ID NLM: 0045503
Informations de publication
Date de publication:
01 11 2019
01 11 2019
Historique:
received:
08
05
2019
revised:
21
07
2019
accepted:
22
07
2019
pubmed:
28
7
2019
medline:
22
10
2020
entrez:
27
7
2019
Statut:
ppublish
Résumé
Although it is known that acetylcholine acting through M1 muscarinic receptors (M1Rs) is essential for memory consolidation in the anterior basolateral nucleus of the amygdala (BLa), virtually nothing is known about the circuits involved. In the hippocampus M1R activation facilitates long-term potentiation (LTP) by potentiating NMDA glutamate receptor (NMDAR) currents. The majority of NMDAR+ profiles in the BLa are spines. Since about half of dendritic spines of BLa pyramidal neurons (PNs) receiving glutamatergic inputs are M1R-immunoreactive (M1R+) it is possible that the role of M1Rs in BLa mnemonic functions also involves potentiation of NMDAR currents in spines. However, the finding that only about half of BLa spines are M1R+ suggests that this proposed mechanism may only apply to a subset of glutamatergic inputs. As a first step in the identification of differential glutamatergic inputs to M1R+ spines in the BLa, the present electron microscopic study used antibodies to two different vesicular glutamate transporter proteins (VGluTs) to label two different subsets of glutamatergic inputs to M1R+ spines. These inputs are largely complimentary with VGluT1+ inputs arising mainly from cortical structures and the basolateral nucleus, and VGluT2+ inputs arising mainly from the thalamus. It was found that about one-half of the spines that were postsynaptic to VGluT1+ or VGluT2+ terminals were M1R+. In addition, a subset of the VGluT1+ or VGluT2+ axon terminals were M1R+, including those that synapsed with M1R+ spines. These results suggest that acetylcholine can modulate glutamatergic inputs to BLa spines by presynaptic as well as postsynaptic M1R-mediated mechanisms.
Identifiants
pubmed: 31348911
pii: S0006-8993(19)30403-2
doi: 10.1016/j.brainres.2019.146349
pmc: PMC6755062
mid: NIHMS1536408
pii:
doi:
Substances chimiques
Receptor, Muscarinic M1
0
Slc17a6 protein, mouse
0
Slc17a7 protein, mouse
0
Vesicular Glutamate Transport Protein 1
0
Vesicular Glutamate Transport Protein 2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
146349Subventions
Organisme : NIMH NIH HHS
ID : R01 MH104638
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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