PreS1 peptide-functionalized gold nanostructures with SERRS tags for efficient liver cancer cell targeting.
Animals
Antigens, Neoplasm
/ metabolism
Carcinoma, Hepatocellular
/ drug therapy
Drug Delivery Systems
Gold
/ chemistry
Hep G2 Cells
Hepatitis B Surface Antigens
/ chemistry
Humans
Liver Neoplasms
/ drug therapy
Metal Nanoparticles
/ chemistry
Mice
Mice, Transgenic
Neoplasm Proteins
/ metabolism
Peptides
/ chemistry
Protein Precursors
/ chemistry
Serpins
/ metabolism
Spectrum Analysis, Raman
Gold nanoparticles
HBV PreS1(21–47)
SerpinB3
Surface enhanced Raman spectroscopy
Tumor targeting
Journal
Materials science & engineering. C, Materials for biological applications
ISSN: 1873-0191
Titre abrégé: Mater Sci Eng C Mater Biol Appl
Pays: Netherlands
ID NLM: 101484109
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
23
01
2019
revised:
10
05
2019
accepted:
15
05
2019
entrez:
28
7
2019
pubmed:
28
7
2019
medline:
31
12
2019
Statut:
ppublish
Résumé
Early detection is the most effective mean of improving prognosis for many fatal diseases such as cancer. In this context, the Surface Enhanced Resonance Raman Scattering (SERRS) technique is being proposed as alternative to fluorescent methods in detection of biomarkers, because SERRS nanostructures are bright as fluorescent tags but more stable and clearly detectable using the narrow Raman "fingerprints" of a suitable reporter. Here we show that biocompatible SERRS active gold nanostructures, functionalized with an engineered PreS1 peptide (AuNP@PEG-PreS1), detect the presence of the SerpinB3 antigen overexpressed on liver tumor cells, a biomarker of the onset of liver cell carcinomatous transformation. A proper engineering of the targeting unit, linked to the nanostructure by a polymer chain, affords a sensitivity and specificity larger than 80%, at subnanomolar concentrations. Taking into account the high sensitivity of SERRS and that SB3 overexpression is an early event in liver cell carcinomatous transformation, AuNP@PEG-PreS1 nanostructures could be used in routine diagnostic activities, to improve the accuracy of HCC detection in particular in patients with chronic liver diseases.
Identifiants
pubmed: 31349478
pii: S0928-4931(19)30285-1
doi: 10.1016/j.msec.2019.109762
pii:
doi:
Substances chimiques
Antigens, Neoplasm
0
Hepatitis B Surface Antigens
0
Neoplasm Proteins
0
Peptides
0
Protein Precursors
0
Serpins
0
presurface protein 1, hepatitis B surface antigen
0
squamous cell carcinoma-related antigen
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109762Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.