Cells, drugs and NMR.
Dynamic nuclear polarization
In vivo NMR
In-cell NMR
Metabolomics
On-cell NMR
Solid-state NMR
Whole-cell NMR
Journal
Journal of magnetic resonance (San Diego, Calif. : 1997)
ISSN: 1096-0856
Titre abrégé: J Magn Reson
Pays: United States
ID NLM: 9707935
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
18
05
2019
revised:
08
06
2019
accepted:
08
07
2019
pubmed:
31
7
2019
medline:
31
10
2020
entrez:
31
7
2019
Statut:
ppublish
Résumé
Nuclear magnetic resonance (NMR) spectroscopy is a versatile tool for investigating cellular structures and their compositions. While in vivo and whole-cell NMR have a long tradition in cell-based approaches, high-resolution in-cell NMR spectroscopy is a new addition to these methods. In recent years, technological advancements in multiple areas provided converging benefits for cellular MR applications, especially in terms of robustness, reproducibility and physiological relevance. Here, we review the use of cellular NMR methods for drug discovery purposes in academia and industry. Specifically, we discuss how developments in NMR technologies such as miniaturized bioreactors and flow-probe perfusion systems have helped to consolidate NMR's role in cell-based drug discovery efforts.
Identifiants
pubmed: 31358370
pii: S1090-7807(19)30144-2
doi: 10.1016/j.jmr.2019.07.018
pii:
doi:
Substances chimiques
Pharmaceutical Preparations
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
202-212Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.