A MYST family histone acetyltransferase, MoSAS3, is required for development and pathogenicity in the rice blast fungus.
Epistasis, Genetic
Fungal Proteins
/ metabolism
Gene Deletion
Gene Expression Regulation, Fungal
Gene Ontology
Histone Acetyltransferases
/ chemistry
Hyphae
/ growth & development
Magnaporthe
/ enzymology
Oryza
/ microbiology
Plant Diseases
/ microbiology
Protein Domains
Reproduction, Asexual
Spores, Fungal
/ growth & development
MoSAS3
development
histone acetyltransferase
pathogenicity
rice blast
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
pubmed:
1
8
2019
medline:
9
7
2020
entrez:
1
8
2019
Statut:
ppublish
Résumé
Histone acetylation has been established as a principal epigenetic regulatory mechanism in eukaryotes. Sas3, a histone acetyltransferase belonging to the largest family of acetyltransferase, MYST, is the catalytic subunit of a conserved histone acetyltransferase complex. To date, the functions of Sas3 and its orthologues have been extensively studied in yeast, humans and flies in relation to global acetylation and transcriptional regulation. However, its precise impact on development and pathogenicity in fungal plant pathogens has yet to be elucidated. Considering the importance of Sas3 in H3K14 acetylation, here we investigate the roles of its orthologue in the rice blast fungus, Magnaporthe oryzae (Pyricularia oryzae). Unlike a previously reported Sas3 deletion in yeast, which led to no remarkable phenotypic changes, we found that MoSAS3 deletion alone had a profound effect on fungal growth and development, including asexual reproduction, germination and appressorium formation in M. oryzae. Such defects in pre-penetration development resulted in complete loss of pathogenicity in the deletion mutant. Furthermore, genetic analysis of MoSAS3 and MoGCN5 encoding a Gcn5-related N-acetyltransferase family histone acetyltransferase suggested that two conserved components of histone acetylation are integrated differently into epigenetic regulatory mechanisms in the yeast and a filamentous fungus. RNA-seq analysis of ΔMosas3 showed two general trends: many DNA repair and DNA damage response genes are up-regulated, while carbon and nitrogen metabolism genes are down-regulated in ΔMosas3. Our work demonstrates the importance of MYST family histone acetyltransferase as a developmental regulator and illuminates a degree of functional variation in conserved catalytic subunits among different fungal species.
Identifiants
pubmed: 31364260
doi: 10.1111/mpp.12856
pmc: PMC6804344
doi:
Substances chimiques
Fungal Proteins
0
Histone Acetyltransferases
EC 2.3.1.48
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1491-1505Informations de copyright
© 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
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