Rebuilding core abscisic acid signaling pathways of Arabidopsis in yeast.
ABF
RCAR/PYL/PYR1
SnRK2
abiotic stress
abscisic acid
clade A PP2C
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
02 09 2019
02 09 2019
Historique:
received:
21
02
2019
revised:
01
07
2019
accepted:
09
07
2019
pubmed:
2
8
2019
medline:
24
12
2019
entrez:
2
8
2019
Statut:
ppublish
Résumé
The phytohormone abscisic acid (ABA) regulates plant responses to abiotic stress, such as drought and high osmotic conditions. The multitude of functionally redundant components involved in ABA signaling poses a major challenge for elucidating individual contributions to the response selectivity and sensitivity of the pathway. Here, we reconstructed single ABA signaling pathways in yeast for combinatorial analysis of ABA receptors and coreceptors, downstream-acting SnRK2 protein kinases, and transcription factors. The analysis shows that some ABA receptors stimulate the pathway even in the absence of ABA and that SnRK2s are major determinants of ABA responsiveness by differing in the ligand-dependent control. Five SnRK2s, including SnRK2.4 known to be active under osmotic stress in plants, activated ABA-responsive transcription factors and were regulated by ABA receptor complexes in yeast. In the plant tissue, SnRK2.4 and ABA receptors competed for coreceptor interaction in an ABA-dependent manner consistent with a tight integration of SnRK2.4 into the ABA signaling pathway. The study establishes the suitability of the yeast system for the dissection of core signaling cascades and opens up future avenues of research on ligand-receptor regulation.
Identifiants
pubmed: 31368592
doi: 10.15252/embj.2019101859
pmc: PMC6717914
doi:
Substances chimiques
Arabidopsis Proteins
0
SnRK2 protein, Arabidopsis
0
Abscisic Acid
72S9A8J5GW
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e101859Subventions
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : GR938
Pays : International
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : SFB924
Pays : International
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 The Authors. Published under the terms of the CC BY 4.0 license.
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