A portable epigenetic switch for bistable gene expression in bacteria.
Anti-Bacterial Agents
/ pharmacology
Biosensing Techniques
/ methods
DNA Methylation
Disk Diffusion Antimicrobial Tests
Drug Resistance, Bacterial
/ genetics
Epigenesis, Genetic
Escherichia coli
/ drug effects
Gene Expression Regulation, Bacterial
Genes, Bacterial
/ genetics
Mutagenesis, Site-Directed
Mutation
Operon
/ genetics
Plasmids
/ genetics
Promoter Regions, Genetic
/ genetics
Salmonella typhimurium
/ drug effects
Synthetic Biology
/ methods
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
02 08 2019
02 08 2019
Historique:
received:
15
05
2019
accepted:
22
07
2019
entrez:
4
8
2019
pubmed:
4
8
2019
medline:
11
11
2020
Statut:
epublish
Résumé
We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics.
Identifiants
pubmed: 31375711
doi: 10.1038/s41598-019-47650-2
pii: 10.1038/s41598-019-47650-2
pmc: PMC6677893
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11261Commentaires et corrections
Type : ErratumIn
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