Study the effect of trypsin enzyme activity on the screening of applying frontal affinity chromatography.

Alkaloids / chemistry Chromatography, Affinity Genetic Engineering Glycerol / chemistry Hot Temperature Kinetics Magnetic Resonance Spectroscopy Microscopy, Electron, Scanning Peptides / chemistry Quinolizines / chemistry Silanes / chemistry Solvents / chemistry Spectrophotometry, Ultraviolet Spectroscopy, Fourier Transform Infrared Trypsin / chemistry Matrines

Frontal affinity chromatography Screening Trypsin enzyme activity

Journal

International journal of biological macromolecules

ISSN: 1879-0003

Titre abrégé: Int J Biol Macromol

Pays: Netherlands

ID NLM: 7909578

Informations de publication

Date de publication:
15 Oct 2019

Historique:

received: 25 06 2019

revised: 30 07 2019

accepted: 31 07 2019

pubmed: 5 8 2019

medline: 13 5 2020

entrez: 5 8 2019

Statut: ppublish

Résumé

Frontal affinity chromatography (FAC) combined with enzyme has been widely used for drug screening. In this paper, the effect of target enzyme activity on screening of bioactive compounds was studied through applying FAC. Trypsin with different degree of inactivation were prepared as target enzyme by thermal denaturation. Their primary structure was identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and use Fourier transform infrared (FTIR) and ultraviolet-visible (UV-vis) spectroscopy to detect group structure. Ultimately, it was found that the main structure of enzyme with decreased activity remained unchanged. The oxymatrine and matrine which can interact with trypsin were selected to study their binding to trypsin with different activities in FAC. The results showed that oxymatrine and matrine had a significant difference in the breakthrough volume among seven kinds of columns prepared by trypsins with different activities, at the different concentration. It indicated that trypsins with different activities in FAC could combine with oxymatrine and matrine. The binding constant (K

Identifiants

DOI: 10.1016/j.ijbiomac.2019.07.218 PMID: 31377290

pubmed: 31377290

pii: S0141-8130(19)34777-4

doi: 10.1016/j.ijbiomac.2019.07.218

pii:

doi:

Substances chimiques

Alkaloids 0

Peptides 0

Quinolizines 0

Silanes 0

Solvents 0

tetraethoxysilane 42064KRE49

oxymatrine 85U4C366QS

Trypsin EC 3.4.21.4

Glycerol PDC6A3C0OX

Matrines 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

740-751

Study the effect of trypsin enzyme activity on the screening of applying frontal affinity chromatography.

Journal

Informations de publication

Résumé

Identifiants

Substances chimiques

Types de publication

Langues

Sous-ensembles de citation

Pagination

Informations de copyright

Auteurs

JunQing Qian (J)

ChangYan Zhao (C)

Jun Tong (J)

ShengLan Jiang (S)

Zheng Zhang (Z)

ShiYong Lu (S)

Hui Guo (H)

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Classifications MeSH