A Trp-574-Leu mutation in the acetolactate synthase (ALS) gene of Lithospermum arvense L. confers broad-spectrum resistance to ALS inhibitors.
ALS
Gene mutation
Herbicide resistance
Lithospermum arvense
Journal
Pesticide biochemistry and physiology
ISSN: 1095-9939
Titre abrégé: Pestic Biochem Physiol
Pays: United States
ID NLM: 1301573
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
29
11
2018
revised:
28
03
2019
accepted:
01
04
2019
entrez:
6
8
2019
pubmed:
6
8
2019
medline:
24
8
2019
Statut:
ppublish
Résumé
Lithospermum arvense is a troublesome dicotyledonous winter annual weed of wheat in China. A L. arvense population (HN01) suspected of being resistant to acetolactate synthase (ALS) inhibitors was found in Henan Province, China. This study aimed to testify the sensitivity of this HN01 population to eight herbicides from 3 different modes of action, and to explore the potential target-site-resistance mechanism to tribenuron-methyl. The whole-plant bioassays indicated that the population was highly resistant to tribenuron-methyl (SU, 350-fold), pyrithiobac sodium (PTB, 151-fold), pyroxsulam (TP, 62.7-fold), florasulam (TP, 80.6-fold), and imazethapyr (IMI, 136-fold), but was sensitive to carfentrazone-ethyl and fluroxypyr-meptyl. ALS gene sequencing revealed that the Trp (TGG) was substituted by Leu (TTG) at codon 574 in resistant plants. In in vitro ALS assays, the concentration of tribenuron-methyl required to inhibit 50% ALS activity (I
Identifiants
pubmed: 31378346
pii: S0048-3575(18)30605-9
doi: 10.1016/j.pestbp.2019.04.001
pii:
doi:
Substances chimiques
Arylsulfonates
0
Benzoates
0
Herbicides
0
Nicotinic Acids
0
Plant Proteins
0
Pyrimidines
0
Sulfonamides
0
florasulam
00A64ZX8NB
metsulfuron methyl
2589ET7417
pyrithiobac sodium
5M13LUJ9WY
imazethapyr
72T2IN94I4
Acetolactate Synthase
EC 2.2.1.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12-17Informations de copyright
Copyright © 2019. Published by Elsevier Inc.