Phylogenetic characterization of rhinoviruses from infants in Sarlahi, Nepal.
Acute Disease
/ epidemiology
Family Characteristics
Genetic Variation
Humans
Infant
Infant, Newborn
Nepal
/ epidemiology
Phylogeny
Picornaviridae Infections
/ epidemiology
Respiratory Tract Infections
/ epidemiology
Rhinovirus
/ classification
Rural Population
/ statistics & numerical data
Sequence Analysis, DNA
genotypes
infants
respiratory illness
rhinoviruses
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
30
05
2019
accepted:
01
08
2019
pubmed:
8
8
2019
medline:
16
7
2020
entrez:
8
8
2019
Statut:
ppublish
Résumé
Rhinoviruses (RVs), the most common causes of acute respiratory infections in young children and infants, are highly diverse genetically. To characterize the RV types detected with respiratory illness episodes in infants in Nepal. Infants born to women enrolled in a randomized trial of maternal influenza immunization in rural, southern Nepal were followed with household-based weekly surveillance until 180 days of age. Infants with respiratory symptoms had nasal swabs tested for twelve respiratory viruses. A subset with RV alone was selected for sequencing of the VP4/2 gene to identify RV types. Among 547 RV-only positive illnesses detected from December 2012 to April 2014, 285 samples (52%) were sequenced. RV-A, B, and C species were detected in 193 (68%), 18 (6%), and 74 (26%) specimens, respectively. A total of 94 unique types were identified from the sequenced samples, including 52 RV-A, 11 RV-B, and 31 RV-C. Multiple species and types circulated simultaneously throughout the study period. No seasonality was observed. The median ages at illness onset were 88, 104, and 88 days for RV-A, B, and C, respectively. The median polymerase chain reaction cycle threshold values did not differ between RV species. No differences between RV species were observed for reported respiratory symptoms, including pneumonia, or for medical care-seeking. Among very young, symptomatic infants in rural Nepal, all three species and many types of RV were identified; RV-A was detected most frequently. There was no association between RV species and disease severity.
Identifiants
pubmed: 31389049
doi: 10.1002/jmv.25563
pmc: PMC6800797
mid: NIHMS1045053
doi:
Types de publication
Journal Article
Randomized Controlled Trial
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2108-2116Subventions
Organisme : NIAID NIH HHS
ID : K23 AI103105
Pays : United States
Informations de copyright
© 2019 Wiley Periodicals, Inc.
Références
J Med Virol. 2013 Sep;85(9):1645-51
pubmed: 23780670
J Clin Virol. 2014 Dec;61(4):558-64
pubmed: 25453332
J Clin Microbiol. 2009 Dec;47(12):3958-67
pubmed: 19828751
J Clin Microbiol. 2009 Dec;47(12):4131-2
pubmed: 19794045
Viruses. 2012 Feb;4(2):200-10
pubmed: 22470832
J Med Virol. 2017 Apr;89(4):582-588
pubmed: 27677921
Clin Microbiol Infect. 2016 Dec;22(12):1002.e9-1002.e14
pubmed: 27554204
J Clin Virol. 2005 Aug;33(4):299-305
pubmed: 16036180
Clin Microbiol Rev. 2013 Jan;26(1):135-62
pubmed: 23297263
Blood. 2010 Mar 11;115(10):2088-94
pubmed: 20042728
Eur J Clin Microbiol Infect Dis. 2013 Jul;32(7):947-54
pubmed: 23435753
BMC Pregnancy Childbirth. 2015 Feb 20;15:40
pubmed: 25879974
Med Microbiol Immunol. 2013 Aug;202(4):303-11
pubmed: 23625169
Influenza Other Respir Viruses. 2014 Sep;8(5):567-73
pubmed: 24990601
Lancet Infect Dis. 2017 Sep;17(9):981-989
pubmed: 28522338
PLoS One. 2013 Nov 19;8(11):e80614
pubmed: 24260436
J Clin Virol. 2013 Dec;58(4):671-7
pubmed: 23932333
J Clin Virol. 2015 Jan;62:25-31
pubmed: 25542466
Front Microbiol. 2018 Aug 07;9:1836
pubmed: 30131797
J Med Virol. 2019 Dec;91(12):2108-2116
pubmed: 31389049
J Virol. 2010 Aug;84(15):7418-26
pubmed: 20375160
Am J Respir Crit Care Med. 2012 Nov 1;186(9):886-91
pubmed: 22923659
Influenza Other Respir Viruses. 2016 May;10(3):185-91
pubmed: 26822469
J Infect Dis. 2017 Nov 27;216(9):1104-1111
pubmed: 28968668
Am J Clin Pathol. 2014 Aug;142(2):165-72
pubmed: 25015856
J Gen Virol. 2013 Aug;94(Pt 8):1791-1806
pubmed: 23677786
PLoS One. 2018 Jul 3;13(7):e0198624
pubmed: 29969445
J Clin Virol. 2013 Mar;56(3):255-9
pubmed: 23290385
J Clin Virol. 2012 Apr;53(4):280-4
pubmed: 22265827
Eur Respir J. 2011 May;37(5):1037-42
pubmed: 20693244
J Infect Dis. 2018 Aug 14;218(6):848-855
pubmed: 29684211
Curr Opin Pediatr. 2008 Feb;20(1):67-71
pubmed: 18197042
Infect Genet Evol. 2014 Jan;21:90-102
pubmed: 24200591
Chin Med J (Engl). 2016 Jul 5;129(13):1513-8
pubmed: 27364785
Clin Microbiol Infect. 2016 Aug;22(8):736.e9-736.e15
pubmed: 27265373
J Clin Microbiol. 2006 Jul;44(7):2382-8
pubmed: 16825353
J Pediatric Infect Dis Soc. 2020 Feb 28;9(1):21-29
pubmed: 30423150
Open Forum Infect Dis. 2017 Apr 06;4(2):ofx073
pubmed: 28584855
Influenza Other Respir Viruses. 2016 Nov;10(6):493-503
pubmed: 27383422
Pediatrics. 2007 Jan;119(1):e70-6
pubmed: 17130280