Chemical profiling and pharmaco-toxicological activity of Origanum sipyleum extracts: Exploring for novel sources for potential therapeutic agents.
Origanum sipyleum
anti-inflammatory
antibacterial-antifungal
enzyme inhibition
free radicals
phenolic compounds
Journal
Journal of food biochemistry
ISSN: 1745-4514
Titre abrégé: J Food Biochem
Pays: United States
ID NLM: 7706045
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
18
05
2019
revised:
24
06
2019
accepted:
28
06
2019
pubmed:
9
8
2019
medline:
22
9
2020
entrez:
9
8
2019
Statut:
ppublish
Résumé
The phytochemical, antiradical, and enzyme inhibition profile of three solvent extracts (ethyl acetate, methanol, water) of Origanum sipyleum were assessed. We also performed a pharmacological study in order to explore protective effects induced by extracts in inflamed colon. LC-MS analysis revealed that the extracts contained different classes of phenolics. The aqueous extract showed the highest antioxidant and acetylcholinesterase (AChE) inhibitory effects. Total phenol and flavonoid contents were highest in aqueous and ethyl acetate extract, respectively. All extracts were effective in reducing colon pro-oxidant and pro-inflammatory biomarkers. The extracts revealed also able to inhibit fungal and bacterial species involved in ulcerative colitis, including Candida albicans, Candida tropicalis, Staphylococcus aureus, and Staphylococcus thyphimurium. Finally, we also showed the antiproliferative effects exerted by the EA extracts on human colon cancer HCT116 cell line. Concluding, our results indicated that O. sipyleum extracts displayed promising therapeutic properties which warrants further validation. PRACTICAL APPLICATIONS: The present phytochemical and biological studies, including antioxidant, anti-inflammatory, and antimicrobic assessments, showed significant protective effects exerted by O. sipyleum extracts in an experimental model of ulcerative colitis. The results are intriguing and suggest potential applications O. sipyleum extracts as sources of natural agents for the management of clinical symptoms related to ulcerative colitis, characterized by increased burden of oxidative stress and microbiome dysbiosis.
Substances chimiques
Acetates
0
Anti-Inflammatory Agents
0
Antioxidants
0
Cholinesterase Inhibitors
0
Flavonoids
0
Phenols
0
Phytochemicals
0
Plant Extracts
0
Solvents
0
ethyl acetate
76845O8NMZ
Acetylcholinesterase
EC 3.1.1.7
Methanol
Y4S76JWI15
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13003Informations de copyright
© 2019 Wiley Periodicals, Inc.
Références
Achitei, D., Ciobica, A., Balan, G., Gologan, E., Stanciu, C., & Stefanescu, G. (2013). Different profile of peripheral antioxidant enzymes and lipid peroxidation in active and non-active inflammatory bowel disease patients. Digestive Diseases and Sciences, 58(5), 1244-1249. https://doi.org/10.1007/s10620-012-2510-z
Acosta-Quezada, P. G., Raigón, M. D., Riofrío-Cuenca, T., García-Martínez, M. D., Plazas, M., Burneo, J. I., … Prohens, J. (2015). Diversity for chemical composition in a collection of different varietal types of tree tomato (Solanum betaceum Cav.), an Andean exotic fruit. Food Chemistry, 169, 327-335. https://doi.org/10.1016/j.foodchem.2014.07.152
Baloglu, M. C., Llorent-Martínez, E. J., Aumeeruddy, M. Z., Mahomoodally, M. F., Altunoglu, Y. C., Ustaoglu, B., … Zengin, G. (2019). Multidirectional insights on Chrysophyllum perpulchrum leaves and stem bark extracts: HPLC-ESI-MSn profiles, antioxidant, enzyme inhibitory, antimicrobial and cytotoxic properties. Industrial Crops and Products, 134, 33-42. https://doi.org/10.1016/j.indcrop.2019.03.066
Barbouchi, M., Elamrani, K., El Idrissi, M., & Choukrad, M. (2018). A comparative study on phytochemical screening, quantification of phenolic contents and antioxidant properties of different solvent extracts from various parts of Pistacia lentiscus L. Journal of King Saud University-Science.
Baser, K. H. C. (2002). The Turkish origanum species. Spiridon EK Oregano. The genera Origanum and Lippia (1st ed., pp. 109-126). New York, NY: Taylor & Francis Inc.
Baser, K., Özek, T., Kürkçüoglu, M., & Tümen, G. (1992). Composition of the essential oil of Origanum sipyleum of Turkish origin. Journal of Essential Oil Research, 4(2), 139-142.
Bottari, N. B., Lopes, L. Q. S., Pizzuti, K., Filippi dos Santos Alves, C., Corrêa, M. S., Bolzan, L. P., … Santos, R. C. V. (2017). Antimicrobial activity and phytochemical characterization of Carya illinoensis. Microbial Pathogenesis, 104, 190-195. https://doi.org/10.1016/j.micpath.2017.01.037
Brunetti, L., Orlando, G., Ferrante, C., Recinella, L., Leone, S., Chiavaroli, A., … Vacca, M. (2014). Peripheral chemerin administration modulates hypothalamic control of feeding. Peptides, 51, 115-121. https://doi.org/10.1016/j.peptides.2013.11.007
Bungo, T., Shiraishi, J.-I., Yanagita, K., Ohta, Y., & Fujita, M. (2009). Effect of nociceptin/orphanin FQ on feeding behavior and hypothalamic neuropeptide expression in layer-type chicks. General and Comparative Endocrinology, 163(1-2), 47-51. https://doi.org/10.1016/j.ygcen.2009.03.009
Chandel, P., Rawal, R. K., & Kaur, R. (2018). Natural products and their derivatives as cyclooxygenase-2 inhibitors. Future Medicinal Chemistry, 10(20), 2471-2492. https://doi.org/10.4155/fmc-2018-0120
Charan, J., & Kantharia, N. (2013). How to calculate sample size in animal studies? Journal of Pharmacology & Pharmacotherapeutics, 4(4), 303. https://doi.org/10.4103/0976-500X.119726
Chen, W. P., Jin, G. J., Xiong, Y., Hu, P. F., Bao, J. P., & Wu, L. D. (2018). Rosmarinic acid down-regulates NO and PGE 2 expression via MAPK pathway in rat chondrocytes. Journal of Cellular and Molecular Medicine, 22(1), 346-353.
Chiavaroli, A., Brunetti, L., Orlando, G., Recinella, L., Ferrante, C., Leone, S., … Vacca, M. (2010). Resveratrol inhibits isoprostane production in young and aged rat brain. Journal of Biological Regulators & Homeostatic Agents, 24(4), 441-446.
Cho, W.-S., & Chae, C. (2004). Expression of cyclooxygenase-2 and nitric oxide synthase 2 in swine ulcerative colitis caused by Salmonella typhimurium. Veterinary Pathology, 41(4), 419-423.
Clark, K. A., MohanKumar, S. M., Kasturi, B. S., & MohanKumar, P. (2006). Effects of central and systemic administration of leptin on neurotransmitter concentrations in specific areas of the hypothalamus. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 290(2), R306-R312. https://doi.org/10.1152/ajpregu.00350.2005
Clifford, M. N., Johnston, K. L., Knight, S., & Kuhnert, N. (2003). Hierarchical scheme for LC-MS n identification of chlorogenic acids. Journal of Agricultural and Food Chemistry, 51(10), 2900-2911.
Clifford, M. N., Knight, S., & Kuhnert, N. (2005). Discriminating between the six isomers of dicaffeoylquinic acid by LC-MS n. Journal of Agricultural and Food Chemistry, 53(10), 3821-3832.
Cool, D., Leibach, F., Bhalla, V., Mahesh, V., & Ganapathy, V. (1991). Expression and cyclic AMP-dependent regulation of a high affinity serotonin transporter in the human placental choriocarcinoma cell line (JAR). Journal of Biological Chemistry, 266(24), 15750-15757.
de Camargo, A. C., Regitano-d'Arce, M. A. B., Rasera, G. B., Canniatti-Brazaca, S. G., do Prado-Silva, L., Alvarenga, V. O., … Shahidi, F. (2017). Phenolic acids and flavonoids of peanut by-products: Antioxidant capacity and antimicrobial effects. Food Chemistry, 237, 538-544. https://doi.org/10.1016/j.foodchem.2017.05.046
de Rijke, E., Out, P., Niessen, W. M., Ariese, F., Gooijer, C., & Udo, A. T. (2006). Analytical separation and detection methods for flavonoids. Journal of Chromatography A, 1112(1-2), 31-63. https://doi.org/10.1016/j.chroma.2006.01.019
Dias, H. J., de Melo, N. I., & Crotti, A. E. M. (2012). Electrospray ionization tandem mass spectrometry as a tool for the structural elucidation and dereplication of natural products: An overview. In Tandem mass spectrometry-applications and principles (pp. 601-603): London: InTech.
Durmuşkahya, C., Alp, H., Hortooğlu, Z. S., Toktas, Ü., & Kayalar, H. (2016). X-ray fluorescence spectroscopic determination of heavy metals and trace elements in aerial parts of Origanum sipyleum L from Turkey. Tropical Journal of Pharmaceutical Research, 15(5), 1013-1015.
Ferrante, C., Orlando, G., Recinella, L., Leone, S., Chiavaroli, A., Di Nisio, C., … Brunetti, L. (2016). Central inhibitory effects on feeding induced by the adipo-myokine irisin. European Journal of Pharmacology, 791, 389-394. https://doi.org/10.1016/j.ejphar.2016.09.011
Ferrante, C., Recinella, L., Ronci, M., Menghini, L., Brunetti, L., Chiavaroli, A., … Orlando, G. (2019). Multiple pharmacognostic characterization on hemp commercial cultivars: Focus on inflorescence water extract activity. Food and Chemical Toxicology, 125, 452-461. https://doi.org/10.1016/j.fct.2019.01.035
Gao, S., Zhan, Q., Li, J., Yang, Q., Li, X., Chen, W., & Sun, L. (2010). LC-MS/MS method for the simultaneous determination of ethyl gallate and its major metabolite in rat plasma. Biomedical Chromatography, 24(5), 472-478.
Goggins, M. G., Shah, S. A., Goh, J., Cherukuri, A., Weir, D. G., Kelleher, D., & Mahmud, N. (2001). Increased urinary nitrite, a marker of nitric oxide, in active inflammatory bowel disease. Mediators of Inflammation, 10(2), 69-73. https://doi.org/10.1080/09629350120054536
Guo, M., Ding, S., Zhao, C., Gu, X., He, X., Huang, K., … Xu, W. (2015). Red Ginseng and Semen Coicis can improve the structure of gut microbiota and relieve the symptoms of ulcerative colitis. Journal of Ethnopharmacology, 162, 7-13. https://doi.org/10.1016/j.jep.2014.12.029
He, W., Li, Y., Liu, M., Yu, H., Chen, Q., Chen, Y., … Wang, T. (2018). Citrus aurantium L. and its flavonoids regulate TNBS-induced inflammatory bowel disease through anti-inflammation and suppressing isolated jejunum contraction. International Journal of Molecular Sciences, 19(10), 3057-3071.
Herbrechter, R., Ziemba, P. M., Hoffmann, K. M., Hatt, H., Werner, M., & Gisselmann, G. (2015). Identification of Glycyrrhiza as the rikkunshito constituent with the highest antagonistic potential on heterologously expressed 5-HT3A receptors due to the action of flavonoids. Frontiers in Pharmacology, 6, 130. https://doi.org/10.3389/fphar.2015.00130
Hilt, P., Schieber, A., Yildirim, C., Arnold, G., Klaiber, I., Conrad, J., … Carle, R. (2003). Detection of Phloridzin in Strawberries (Fragaria x ananassa Duch.) by HPLC− PDA− MS/MS and NMR Spectroscopy. Journal of Agricultural and Food Chemistry, 51(10), 2896-2899.
Iguidbashian, J. P., Parekh, J. D., Kukrety, S., & Andukuri, V. G. (2018). Campylobacter jejuni and Pseudomonas coinfection in the setting of ulcerative colitis. BMJ Case Reports, 2018, https://doi.org/10.1136/bcr-2018-224941.
Islam, T., Yu, X., & Xu, B. (2016). Phenolic profiles, antioxidant capacities and metal chelating ability of edible mushrooms commonly consumed in China. LWT-Food Science and Technology, 72, 423-431. https://doi.org/10.1016/j.lwt.2016.05.005
Ju, J., Kwak, Y., Hao, X., & Yang, C. S. (2012). Inhibitory effects of calcium against intestinal cancer in human colon cancer cells and ApcMin/+ mice. Nutrition Research and Practice, 6(5), 396-404.
Justesen, U. (2001). Collision-induced fragmentation of deprotonated methoxylated flavonoids, obtained by electrospray ionization mass spectrometry. Journal of Mass Spectrometry, 36(2), 169-178. https://doi.org/10.1002/jms.118
Kamel, K. M., Khalil, I. A., Rateb, M. E., Elgendy, H., & Elhawary, S. (2017). Chitosan-coated cinnamon/oregano-loaded solid lipid nanoparticles to augment 5-fluorouracil cytotoxicity for colorectal cancer: Extract standardization, nanoparticle optimization, and cytotoxicity evaluation. Journal of Agricultural and Food Chemistry, 65(36), 7966-7981. https://doi.org/10.1021/acs.jafc.7b03093
Kannan, N., & Guruvayoorappan, C. (2013). Protective effect of Bauhinia tomentosa on acetic acid induced ulcerative colitis by regulating antioxidant and inflammatory mediators. International Immunopharmacology, 16(1), 57-66. https://doi.org/10.1016/j.intimp.2013.03.008
Karagöz, A., Artun, F. T., Özcan, G., Melikoğlu, G., Anıl, S., Kültür, Ş., & Sütlüpınar, N. (2015). In vitro evaluation of antioxidant activity of some plant methanol extracts. Biotechnology & Biotechnological Equipment, 29(6), 1184-1189.
Kargıoğlu, M., Cenkci, S., Serteser, A., Evliyaoğlu, N., Konuk, M., Kök, M. Ş., & Bağcı, Y. (2008). An ethnobotanical survey of inner-West Anatolia, Turkey. Human Ecology, 36(5), 763-777. https://doi.org/10.1007/s10745-008-9198-x
Khalifa, M. M., Bakr, A. G., & Osman, A. T. (2017). Protective effects of phloridzin against methotrexate-induced liver toxicity in rats. Biomedicine & Pharmacotherapy, 95, 529-535. https://doi.org/10.1016/j.biopha.2017.08.121
Kim, M., Cooke, H. J., Javed, N. H., Carey, H. V., Christofi, F., & Raybould, H. E. (2001). D-glucose releases 5-hydroxytryptamine from human BON cells as a model of enterochromaffin cells. Gastroenterology, 121(6), 1400-1406. https://doi.org/10.1053/gast.2001.29567
Lazarova, I., Zengin, G., Aktumsek, A., Gevrenova, R., Ceylan, R., & Uysal, S. (2014). HPLC-DAD analysis of phenolic compounds and antioxidant properties of Asphodeline lutea roots from Bulgaria and Turkey. Industrial Crops and Products, 61, 438-441. https://doi.org/10.1016/j.indcrop.2014.07.044
Liu, A.-H., Guo, H., Ye, M., Lin, Y.-H., Sun, J.-H., Xu, M., & Guo, D.-A. (2007). Detection, characterization and identification of phenolic acids in Danshen using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry. Journal of Chromatography A, 1161(1-2), 170-182. https://doi.org/10.1016/j.chroma.2007.05.081
Locatelli, M., Ferrante, C., Carradori, S., Secci, D., Leporini, L., Chiavaroli, A., … Martinotti, S. (2017). Optimization of aqueous extraction and biological activity of Harpagophytum procumbens root on ex vivo rat colon inflammatory model. Phytotherapy Research, 31(6), 937-944.
Locatelli, M., Macchione, N., Ferrante, C., Chiavaroli, A., Recinella, L., Carradori, S., … Leone, S. (2018). Graminex pollen: Phenolic pattern, colorimetric analysis and protective effects in immortalized prostate cells (PC3) and rat prostate challenged with LPS. Molecules, 23(5).
Menghini, L., Ferrante, C., Leporini, L., Recinella, L., Chiavaroli, A., Leone, S., … Brunetti, L. (2016). An hydroalcoholic chamomile extract modulates inflammatory and immune response in HT29 cells and isolated rat colon. Phytotherapy Research, 30(9), 1513-1518. https://doi.org/10.1002/ptr.5655
Menghini, L., Leporini, L., Vecchiotti, G., Locatelli, M., Carradori, S., Ferrante, C., … Orlando, G. (2018). Crocus sativus L. stigmas and byproducts: Qualitative fingerprint, antioxidant potentials and enzyme inhibitory activities. Food Research International, 109, 91-98. https://doi.org/10.1016/j.foodres.2018.04.028
Mousavizadeh, K., Rahimian, R., Fakhfouri, G., Aslani, F., & Ghafourifar, P. (2009). Anti-inflammatory effects of 5-HT3 receptor antagonist, tropisetron on experimental colitis in rats. European Journal of Clinical Investigation, 39(5), 375-383.
Nagarjun, S., Dhadde, S. B., Veerapur, V. P., Thippeswamy, B., & Chandakavathe, B. N. (2017). Ameliorative effect of chromium-D-phenylalanine complex on indomethacin-induced inflammatory bowel disease in rats. Biomedicine & Pharmacotherapy, 89, 1061-1066. https://doi.org/10.1016/j.biopha.2017.02.042
Nagib, M. M., Tadros, M. G., ELSayed, M. I., & Khalifa, A. E. (2013). Anti-inflammatory and anti-oxidant activities of olmesartan medoxomil ameliorate experimental colitis in rats. Toxicology and Applied Pharmacology, 271(1), 106-113. https://doi.org/10.1016/j.taap.2013.04.026
Nakiboglu, M., Urek, R. O., Kayali, H. A., & Tarhan, L. (2007). Antioxidant capacities of endemic Sideritis sipylea and Origanum sipyleum from Turkey. Food Chemistry, 104(2), 630-635. https://doi.org/10.1016/j.foodchem.2006.12.012
Ohikhena, F. U., Wintola, O. A., & Afolayan, A. J. (2016). Research article toxicity assessment of different solvent extracts of the medicinal plant, phragmanthera capitata (Sprengel) Balle on Brine Shrimp (Artemia salina). International Journal of Pharmacology, 12, 701-710. https://doi.org/10.3923/ijp.2016.701.710
Oluk, A., Cakır, A., Yasa, I., Capanlar, S., & Kirmizigul, S. (2013). Comparison of the antimicrobial activity and essential oil content of wild and micropropagated Origanum sipyleum L.: A medicinal herb native to Turkey. Journal of Medicinal Plants Research, 7(6), 230-233.
Ozbilgin, A., Durmuskahya, C., Kayalar, H., Ertabaklar, H., Gunduz, C., Ural, I. O., … Ozbel, Y. (2014). Antileishmanial activity of selected Turkish medicinal plants. Tropical Journal of Pharmaceutical Research, 13(12), 2047-2055. https://doi.org/10.4314/tjpr.v13i12.15
Ozkan, G., & Ozcan, M. (2006). Antioxidant tocopherol constituents from some medicinal and aromatic plants. Chemistry of Natural Compounds, 42(5), 604-605. https://doi.org/10.1007/s10600-006-0225-0
Ozkan, G., Sagdic, O., Ekici, L., Ozturk, I., & Ozcan, M. M. (2007). Phenolic compounds of Origanum sipyleum L. extract, and its antioxidant and antibacterial activities. Journal of Food Lipids, 14(2), 157-169.
Regmi, S. C., Park, S.-Y., Ku, S. K., & Kim, J.-A. (2014). Serotonin regulates innate immune responses of colon epithelial cells through Nox2-derived reactive oxygen species. Free Radical Biology and Medicine, 69, 377-389. https://doi.org/10.1016/j.freeradbiomed.2014.02.003
Rocha, J., Eduardo-Figueira, M., Barateiro, A., Fernandes, A., Brites, D., Bronze, R., … Freitas, M. (2015). Anti-inflammatory effect of rosmarinic acid and an extract of Rosmarinus officinalis in rat models of local and systemic inflammation. Basic & Clinical Pharmacology & Toxicology, 116(5), 398-413.
Saijo, F., Milsom, A. B., Bryan, N. S., Bauer, S. M., Vowinkel, T., Ivanovic, M., … Feelisch, M. (2010). On the dynamics of nitrite, nitrate and other biomarkers of nitric oxide production in inflammatory bowel disease. Nitric Oxide, 22(2), 155-167. https://doi.org/10.1016/j.niox.2009.11.009
Taniguchi, M., Ochiai, A., Namae, T., Saito, K., Kato, T., Saitoh, E., & Tanaka, T. (2018). The antimicrobial and anti-endotoxic peptide AmyI-1-18 from rice α-amylase and its [N3L] analog promote angiogenesis and cell migration. Peptides, 104, 78-84. https://doi.org/10.1016/j.peptides.2018.04.017
Taşcıoğlu, T., Sadıkoğlu, N., Doğanlar, S., & Frary, A. (2018). Molecular genetic diversity in the Origanum genus: EST-SSR and SRAP marker analyses of the 22 species in eight sections that naturally occur in Turkey. Industrial Crops and Products, 123, 746-761. https://doi.org/10.1016/j.indcrop.2018.07.027
Taviano, M. F., Marino, A., Trovato, A., Bellinghieri, V., Melchini, A., Dugo, P., … Güvenç, A. (2013). Juniperus oxycedrus L. subsp. oxycedrus and Juniperus oxycedrus L. subsp. macrocarpa (Sibth. & Sm.) Ball. “berries” from Turkey: Comparative evaluation of phenolic profile, antioxidant, cytotoxic and antimicrobial activities. Food and Chemical Toxicology, 58, 22-29.
Toncer, O., Karaman, S., Kizil, S., & Diraz, E. (2009). Changes in essential oil composition of oregano (Origanum onites L.) due to diurnal variations at different development stages. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37(2), 177-181.
Trojanowska, D., Zwolinska-Wcislo, M., Tokarczyk, M., Kosowski, K., Mach, T., & Budak, A. (2010). The role of Candida in inflammatory bowel disease. Estimation of transmission of C. albicans fungi in gastrointestinal tract based on genetic affinity between strains. Medical Science Monitor, 16(10), CR451-CR457.
Unal, B. T., Guvensen, A., Dereboylu, A. E., & Ozturk, M. (2013). Variations in the proline and total protein contents in Origanum sipyleum L. from different altitudes of spil mountain Turkey. Pakistan Journal of Botany, 45(S1), 571-576.
Uttara, B., Singh, A. V., Zamboni, P., & Mahajan, R. (2009). Oxidative stress and neurodegenerative diseases: A review of upstream and downstream antioxidant therapeutic options. Current Neuropharmacology, 7(1), 65-74.
Uysal, S., Zengin, G., Locatelli, M., Bahadori, M. B., Mocan, A., Bellagamba, G. … Aktumsek, A. (2017). Cytotoxic and enzyme inhibitory potential of two Potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition. Frontiers in Pharmacology, 8, 290.
Wang, X., Tian, G., Duan, Q., Wu, D., Shao, J., Wang, T., & Wang, C. (2018). Therapeutic potential of n-butanol extract of Pulsatilla decoction in a murine model of ulcerative colitis induced by DSS combined with Candida albicans colonization. Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China Journal of Chinese Materia Medica, 43(14), 2979-2984.
Wu, S., Li, J., Wang, Q., Cao, J., Yu, H., Cao, H., & Xiao, J. (2017). Chemical composition, antioxidant and anti-tyrosinase activities of fractions from Stenoloma chusanum. Industrial Crops and Products, 107, 539-545. https://doi.org/10.1016/j.indcrop.2017.04.033
Zengin, G., Aktumsek, A., Ceylan, R., Uysal, S., Mocan, A., Guler, G. O., …Soković, M. (2017). Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). Food & Function, 8(3), 1152-1165.
Zengin, G., Locatelli, M., Stefanucci, A., Macedonio, G., Novellino, E., Mirzaie, S., … Orlando, G. (2017). Chemical characterization, antioxidant properties, anti-inflammatory activity, and enzyme inhibition of Ipomoea batatas L. leaf extracts. International Journal of Food Properties, 20(Suppl 2), 1907-1919.
Zhang, W., Dong, Z., Chang, X., Zhang, C., Rong, G., Gao, X., … Rong, Y. (2018). Protective effect of the total flavonoids from Apocynum venetum L. on carbon tetrachloride-induced hepatotoxicity in vitro and in vivo. Journal of Physiology and Biochemistry, 74(2), 301-312.
Zhang, X.-L., Guo, Y.-S., Wang, C.-H., Li, G.-Q., Xu, J.-J., Chung, H. Y., … Wang, G.-C. (2014). Phenolic compounds from Origanum vulgare and their antioxidant and antiviral activities. Food Chemistry, 152, 300-306. https://doi.org/10.1016/j.foodchem.2013.11.153
Zheleva-Dimitrova, D., Gevrenova, R., Zaharieva, M. M., Najdenski, H., Ruseva, S., Lozanov, V., … Mitev, V. (2017). HPLC-UV and LC-MS analyses of acylquinic acids in Geigeria alata (DC) Oliv. & Hiern. and their contribution to antioxidant and antimicrobial capacity. Phytochemical Analysis, 28(3), 176-184.