Aged fingerprints for DNA profile: First report of successful typing.

DNA / isolation & purification DNA Fingerprinting / methods Dermatoglyphics Electrophoresis, Capillary Humans Microsatellite Repeats Powders Real-Time Polymerase Chain Reaction Regression Analysis Specimen Handling / methods
Dactyloscopic powder Fingerprint Genotyping Short tandem repeat

Journal

Forensic science international
ISSN: 1872-6283
Titre abrégé: Forensic Sci Int
Pays: Ireland
ID NLM: 7902034

Informations de publication

Date de publication:
Sep 2019
Historique:
received: 17 04 2019
revised: 12 06 2019
accepted: 07 07 2019
pubmed: 9 8 2019
medline: 27 11 2019
entrez: 9 8 2019
Statut: ppublish

Résumé

Fingerprint detection and Short Tandem Repeat (STR) typing are two approaches used for identification of individuals. The main goal of forensic laboratories is the development of a standardized protocol to obtain an STR profile from latent fingerprints, by typing the DNA transferred onto touched objects. The results obtained in this field derive from studies conducted under controlled laboratory conditions. Here, for the first time, we report two different profiles obtained by DNA purified by latent fingerprints enhanced with dactyloscopic powders at a crime scene, 14 years previously. DNA extraction phase was optimized to improve removal of powder and automatically conducted. Despite the low concentration of purified DNA, it was not degraded. Even if quality of the profile is influenced by several factors such as the method of acquisition and storage conditions of the fingerprint, results obtained are adequately informative and could be uploaded to the CODIS database.

Identifiants

DOI: 10.1016/j.forsciint.2019.109905 PMID: 31394460
pubmed: 31394460
pii: S0379-0738(19)30318-4
doi: 10.1016/j.forsciint.2019.109905
pii:
doi:

Substances chimiques

Powders 0
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109905

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Carlo Giovanni Romano (CG)

Department of Scientific Investigation (R.I.S.), Division of Biology, Carabinieri, Monsignor D'Arrigo street 5, 98121, Messina, Italy. Electronic address: carlo.romano@carabinieri.it.

Rosaria Mangiaracina (R)

Department of Scientific Investigation (R.I.S.), Division of Biology, Carabinieri, Monsignor D'Arrigo street 5, 98121, Messina, Italy. Electronic address: rosaria.mangiaracina@carabinieri.it.

Luigi Donato (L)

Department of Biomedical and Dental Science and of Morphological and Functional Images, University of Messina, Consolare Valeria Street 1, 98125 Messina, Italy; Department of Vanguard Medicine and Therapies, Biomolecular Strategies and Neuroscience, I.E.ME.S.T., Michele Miraglia Street 20, 90139 Palermo, Italy. Electronic address: ldonato@unime.it.

Rosalia D'Angelo (R)

Department of Biomedical and Dental Science and of Morphological and Functional Images, University of Messina, Consolare Valeria Street 1, 98125 Messina, Italy; Department of Vanguard Medicine and Therapies, Biomolecular Strategies and Neuroscience, I.E.ME.S.T., Michele Miraglia Street 20, 90139 Palermo, Italy. Electronic address: rdangelo@unime.it.

Concetta Scimone (C)

Department of Biomedical and Dental Science and of Morphological and Functional Images, University of Messina, Consolare Valeria Street 1, 98125 Messina, Italy; Department of Vanguard Medicine and Therapies, Biomolecular Strategies and Neuroscience, I.E.ME.S.T., Michele Miraglia Street 20, 90139 Palermo, Italy. Electronic address: cscimone@unime.it.

Antonina Sidoti (A)

Department of Biomedical and Dental Science and of Morphological and Functional Images, University of Messina, Consolare Valeria Street 1, 98125 Messina, Italy; Department of Vanguard Medicine and Therapies, Biomolecular Strategies and Neuroscience, I.E.ME.S.T., Michele Miraglia Street 20, 90139 Palermo, Italy. Electronic address: asidoti@unime.it.

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Classifications MeSH

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questionsmedicales.fr › Administration des services de santé › Organisation et administration › Mesures de sécurité › Identification biométrique › Profilage d'ADN › Aged fingerprints for DNA profile: First...
questionsmedicales.fr › Administration des services de santé › Organisation et administration › Mesures de sécurité › Identification biométrique › Dermatoglyphes › Aged fingerprints for DNA profile: First...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Électrophorèse capillaire › Aged fingerprints for DNA profile: First...
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