Amplicon sequencing provides more accurate microbiome information in healthy children compared to culturing.
Age Factors
Bacteria
/ classification
Bacteriological Techniques
DNA, Bacterial
/ genetics
Feces
/ microbiology
Female
Gastrointestinal Microbiome
Healthy Volunteers
High-Throughput Nucleotide Sequencing
Humans
Hypopharynx
/ microbiology
Infant
Infant, Newborn
Male
Phylogeny
Predictive Value of Tests
Reproducibility of Results
Ribotyping
Bacteria
Microbial communities
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
2019
2019
Historique:
received:
08
03
2019
accepted:
01
07
2019
entrez:
10
8
2019
pubmed:
10
8
2019
medline:
10
8
2019
Statut:
epublish
Résumé
Next-Generation Sequencing (NGS) of 16S rRNA gene is now one of the most widely used application to investigate the microbiota at any given body site in research. Since NGS is more sensitive than traditional culture methods (TCMs), many studies have argued for them to replace TCMs. However, are we really ready for this transition? Here we compare the diagnostic efficiency of the two methods using a large number of samples (
Identifiants
pubmed: 31396571
doi: 10.1038/s42003-019-0540-1
pii: 540
pmc: PMC6683184
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Pagination
291Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing interests.
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