Metagenomic next-generation sequencing aids the diagnosis of viral infections in febrile returning travellers.
Adult
Bacterial Infections
/ diagnosis
Fever
/ virology
High-Throughput Nucleotide Sequencing
/ methods
Humans
Metagenomics
Parasitic Diseases
/ diagnosis
Proof of Concept Study
RNA, Viral
/ genetics
Respiratory Tract Infections
/ blood
Retrospective Studies
Sensitivity and Specificity
Travel
/ statistics & numerical data
Travel-Related Illness
Virus Diseases
/ blood
Viruses
/ genetics
Diagnosis
NGS
Returning travellers
Journal
The Journal of infection
ISSN: 1532-2742
Titre abrégé: J Infect
Pays: England
ID NLM: 7908424
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
08
05
2019
revised:
18
07
2019
accepted:
03
08
2019
pubmed:
10
8
2019
medline:
25
7
2020
entrez:
10
8
2019
Statut:
ppublish
Résumé
Travel-associated infections are challenging to diagnose because of the broad spectrum of potential aetiologies. As a proof-of-principle study, we used MNGS to identify viral pathogens in clinical samples from returning travellers in a single center to explore its suitability as a diagnostic tool. Plasma samples from 40 returning travellers presenting with a fever of ≥38°C were sequenced using MNGS on the Illumina MiSeq platform and compared with standard-of-care diagnostic assays. In total, 11/40 patients were diagnosed with a viral infection. Standard of care diagnostics revealed 5 viral infections using plasma samples; dengue virus 1 (n = 2), hepatitis E (n = 1), Ebola virus (n = 1) and hepatitis A (n = 1), all of which were detected by MNGS. Three additional patients with Chikungunya virus (n = 2) and mumps virus were diagnosed by MNGS only. Respiratory infections detected by nasal/throat swabs only were not detected by MNGS of plasma. One patient had infection with malaria and mumps virus during the same admission. MNGS analysis of plasma samples improves the sensitivity of diagnosis of viral infections and has potential as an all-in-one diagnostic test. It can be used to identify infections that have not been considered by the treating physician, co-infections and new or emerging pathogens. Next generation sequencing (NGS) has potential as an all-in-one diagnostic test. In this study we used NGS to diagnose returning travellers with acute febrile illness in the UK, highlighting cases where the diagnosis was missed using standard methods.
Identifiants
pubmed: 31398374
pii: S0163-4453(19)30242-7
doi: 10.1016/j.jinf.2019.08.003
pmc: PMC6859916
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
383-388Subventions
Organisme : Medical Research Council
ID : MC_UU_12014/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12014/12
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 102789/Z/13/A
Pays : United Kingdom
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
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