Simulation-guided enzyme discovery: A new microbial source of cellobiose 2-epimerase.
Cellobiose 2-epimerase
Lactulose
MD simulation
Thermal stability
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Oct 2019
15 Oct 2019
Historique:
received:
29
05
2019
revised:
13
07
2019
accepted:
07
08
2019
pubmed:
12
8
2019
medline:
9
4
2020
entrez:
12
8
2019
Statut:
ppublish
Résumé
Cellobiose 2-epimerase (CE) is a promising industrial enzyme that can be utilized in the dairy industry. More thermostable CEs from different microorganisms are still needed for a higher lactulose productivity. This study demonstrated the feasibility to use molecular dynamics (MD) simulation as the preliminary computational filter for thermostable enzymes screening. Sequence information of eleven uncharacterized CEs were chosen to be analyzed by MD simulations. The CE from Dictyoglomus thermophilum (Dith-CE) was determined experimentally to be one of the most thermostable CEs with the highest epimerization (160 ± 6.5 U mg
Identifiants
pubmed: 31401280
pii: S0141-8130(19)33910-8
doi: 10.1016/j.ijbiomac.2019.08.075
pmc: PMC9364981
mid: NIHMS1792786
pii:
doi:
Substances chimiques
Cellobiose
16462-44-5
Lactulose
4618-18-2
Racemases and Epimerases
EC 5.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1002-1008Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM068670
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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