Systemic understanding of Lactococcus lactis response to acid stress using transcriptomics approaches.
Acid stress
High-throughput screening
Lactococcus lactis
Metabolic engineering
Transcriptomics
Journal
Journal of industrial microbiology & biotechnology
ISSN: 1476-5535
Titre abrégé: J Ind Microbiol Biotechnol
Pays: Germany
ID NLM: 9705544
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
14
04
2019
accepted:
07
08
2019
pubmed:
16
8
2019
medline:
25
1
2020
entrez:
16
8
2019
Statut:
ppublish
Résumé
During fermentation, acid stress caused by the accumulation of acidic metabolites seriously affects the metabolic activity and production capacity of microbial cells. To elucidate the acid stress-tolerance mechanisms of microbial cells, we performed genome mutagenesis combined with high-throughput technologies to screen acid stress-tolerant strains. Mutant strain Lactococcus lactis WH101 showed a 16,000-fold higher survival rate than that of the parent strain after 5 h of acid shock at pH 4.0 and maintained higher ATP, NH
Identifiants
pubmed: 31414323
doi: 10.1007/s10295-019-02226-x
pii: 10.1007/s10295-019-02226-x
doi:
Substances chimiques
Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1621-1629Subventions
Organisme : the National Key Research and Development Program of China
ID : 2017YFB0308401
Organisme : the Program of Introducing Talents of Discipline to Universities
ID : No. 111-2-06
Organisme : the Open Project of Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University
ID : KLIB-KF201706
Organisme : the National First-class Discipline Program of Light Industry Technology and Engineering
ID : LITE2018-08
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