Compartmentalized Synthesis of Triacylglycerol at the Inner Nuclear Membrane Regulates Nuclear Organization.
Biocatalysis
Cell Compartmentation
Cell Cycle
Cell Nucleolus
/ metabolism
Cell Nucleus Shape
Homeostasis
Imaging, Three-Dimensional
Lipid Droplets
/ metabolism
Nuclear Envelope
/ metabolism
Phospholipids
/ metabolism
Saccharomyces cerevisiae
/ cytology
Saccharomyces cerevisiae Proteins
/ metabolism
Triglycerides
/ biosynthesis
lipid droplet
nuclear membrane
nucleus
phospholipid
triglyceride
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
23 09 2019
23 09 2019
Historique:
received:
02
08
2018
revised:
22
04
2019
accepted:
03
07
2019
pubmed:
20
8
2019
medline:
19
5
2020
entrez:
20
8
2019
Statut:
ppublish
Résumé
Cells dynamically adjust organelle organization in response to growth and environmental cues. This requires regulation of synthesis of phospholipids, the building blocks of organelle membranes, or remodeling of their fatty-acyl (FA) composition. FAs are also the main components of triacyglycerols (TGs), which enable energy storage in lipid droplets. How cells coordinate FA metabolism with organelle biogenesis during cell growth remains unclear. Here, we show that Lro1, an acyltransferase that generates TGs from phospholipid-derived FAs in yeast, relocates from the endoplasmic reticulum to a subdomain of the inner nuclear membrane. Lro1 nuclear targeting is regulated by cell cycle and nutrient starvation signals and is inhibited when the nucleus expands. Lro1 is active at this nuclear subdomain, and its compartmentalization is critical for nuclear integrity. These data suggest that Lro1 nuclear targeting provides a site of TG synthesis, which is coupled with nuclear membrane remodeling.
Identifiants
pubmed: 31422915
pii: S1534-5807(19)30581-7
doi: 10.1016/j.devcel.2019.07.009
pmc: PMC6859503
pii:
doi:
Substances chimiques
Phospholipids
0
Saccharomyces cerevisiae Proteins
0
Triglycerides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
755-766.e6Subventions
Organisme : Wellcome Trust
ID : 093026
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M027252/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 108042
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 086598
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT 107064
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 100140
Pays : United Kingdom
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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