Ariadne's Thread in the Analytical Labyrinth of Membrane Proteins: Integration of Targeted and Shotgun Proteomics for Global Absolute Quantification of Membrane Proteins.

Bacillus subtilis / chemistry Immunoassay Membrane Proteins / analysis Osmotic Pressure Proteomics

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
17 09 2019
Historique:
pubmed: 20 8 2019
medline: 23 9 2020
entrez: 20 8 2019
Statut: ppublish

Résumé

The field of systems biology has been rapidly developing in the past decade. However, the data produced by "omics" approaches is lagging behind the requirements of this field, especially when it comes to absolute abundances of membrane proteins. In the present study, a novel approach for large-scale absolute quantification of this challenging subset of proteins has been established and evaluated using osmotic stress management in the Gram-positive model bacterium

Identifiants

DOI: 10.1021/acs.analchem.9b02869 PMID: 31424929
pubmed: 31424929
doi: 10.1021/acs.analchem.9b02869
doi:

Substances chimiques

Membrane Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11972-11980

Auteurs

Minia Antelo-Varela (M)

Centre of Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics , University of Greifswald , Felix-Hausdorff-Strasse 8 , 17489 Greifswald , Germany.

Jürgen Bartel (J)

Centre of Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics , University of Greifswald , Felix-Hausdorff-Strasse 8 , 17489 Greifswald , Germany.

Ane Quesada-Ganuza (A)

Research & Technology , Novozymes A/S , Krogshoejvej 36 , Bagsværd DK-2880 , Denmark.

Karen Appel (K)

Research & Technology , Novozymes A/S , Krogshoejvej 36 , Bagsværd DK-2880 , Denmark.

Margarita Bernal-Cabas (M)

University Medical Center Groningen, Department of Medical Microbiology , University of Groningen , Hanzeplein 1 , P.O. Box 30001, 9700RB Groningen , The Netherlands.

Thomas Sura (T)

Centre of Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics , University of Greifswald , Felix-Hausdorff-Strasse 8 , 17489 Greifswald , Germany.

Andreas Otto (A)

Centre of Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics , University of Greifswald , Felix-Hausdorff-Strasse 8 , 17489 Greifswald , Germany.

Michael Rasmussen (M)

Research & Technology , Novozymes A/S , Krogshoejvej 36 , Bagsværd DK-2880 , Denmark.

Jan Maarten van Dijl (JM)

University Medical Center Groningen, Department of Medical Microbiology , University of Groningen , Hanzeplein 1 , P.O. Box 30001, 9700RB Groningen , The Netherlands.
ORCID: 0000-0002-5688-8438

Allan Nielsen (A)

Research & Technology , Novozymes A/S , Krogshoejvej 36 , Bagsværd DK-2880 , Denmark.

Sandra Maaß (S)

Centre of Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics , University of Greifswald , Felix-Hausdorff-Strasse 8 , 17489 Greifswald , Germany.

Dörte Becher (D)

Centre of Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics , University of Greifswald , Felix-Hausdorff-Strasse 8 , 17489 Greifswald , Germany.
ORCID: 0000-0002-9630-5735

Articles similaires

A cytoplasmic osmosensing mechanism mediated by molecular crowding-sensitive DCP5.

Zhenyu Wang, Qiuhua Yang, Dan Zhang et al.
1.00
Arabidopsis Arabidopsis Proteins Osmotic Pressure Cytoplasm RNA, Messenger

Expression interplay of genes coding for calcium-binding proteins and transcription factors during the osmotic phase provides insights on salt stress response mechanisms in bread wheat.

Diana Duarte-Delgado, Inci Vogt, Said Dadshani et al.
1.00
Triticum Transcription Factors Gene Expression Regulation, Plant Plant Proteins Salt Stress

Proteomic analysis identified proteins that are differentially expressed in the flavonoid and carotenoid biosynthetic pathways of Camellia Nitidissima flowers.

Xing-Wen Zhou, Xiao-Xia Ye, Bao-Jian Ye et al.
1.00
Camellia Flowers Carotenoids Plant Proteins Flavonoids

Integrated multi-omics revealed that dysregulated lipid metabolism played an important role in RA patients with metabolic diseases.

Xiaoting Zhu, Wubin Long, Jing Zhang et al.
1.00
Humans Arthritis, Rheumatoid Lipid Metabolism Male Female

Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus subtilis › Ariadne's Thread in the Analytical Labyrinth...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Ariadne's Thread in the Analytical Labyrinth...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Ariadne's Thread in the Analytical Labyrinth...
questionsmedicales.fr › Phénomènes chimiques › Perméabilité › Pression osmotique › Ariadne's Thread in the Analytical Labyrinth...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › Ariadne's Thread in the Analytical Labyrinth...