Composition of the mucosa-associated microbiota along the entire gastrointestinal tract of human individuals.
Adenocarcinoma
/ microbiology
Adult
Bacterial Load
Biopsy
Cecal Neoplasms
/ microbiology
Double-Balloon Enteroscopy
Female
Gastric Mucosa
/ microbiology
Gastrointestinal Microbiome
Humans
Male
Middle Aged
Peutz-Jeghers Syndrome
/ microbiology
Polymerase Chain Reaction
RNA, Ribosomal, 16S
/ genetics
Sequence Analysis, RNA
Colonic microflora
colon
gastrointestinal tract
intestinal microbiology
small bowel
Journal
United European gastroenterology journal
ISSN: 2050-6406
Titre abrégé: United European Gastroenterol J
Pays: England
ID NLM: 101606807
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
16
01
2019
accepted:
01
05
2019
entrez:
21
8
2019
pubmed:
21
8
2019
medline:
21
8
2019
Statut:
ppublish
Résumé
Homeostasis of the gastrointestinal tract depends on a healthy bacterial microbiota, with alterations in microbiota composition suggested to contribute to diseases. To unravel bacterial contribution to disease pathology, a thorough understanding of the microbiota of the complete gastrointestinal tract is essential. To date, most microbial analyses have either focused on faecal samples, or on the microbial constitution of one gastrointestinal location instead of different locations within one individual. We aimed to analyse the mucosal microbiome along the entire gastrointestinal tract within the same individuals. Mucosal biopsies were taken from nine different sites in 14 individuals undergoing antegrade and subsequent retrograde double-balloon enteroscopy. The bacterial composition was characterised using 16 S rRNA sequencing with Illumina Miseq. At double-balloon enteroscopy, one individual had a caecal adenocarcinoma and one individual had Peutz-Jeghers polyps. The composition of the microbiota distinctively changed along the gastrointestinal tract with larger bacterial load, diversity and abundance of We show that gastrointestinal location is a larger determinant of mucosal microbial diversity than inter-person differences. These data provide a baseline for further studies investigating gastrointestinal microbiota-related disease.
Sections du résumé
Background
Homeostasis of the gastrointestinal tract depends on a healthy bacterial microbiota, with alterations in microbiota composition suggested to contribute to diseases. To unravel bacterial contribution to disease pathology, a thorough understanding of the microbiota of the complete gastrointestinal tract is essential. To date, most microbial analyses have either focused on faecal samples, or on the microbial constitution of one gastrointestinal location instead of different locations within one individual.
Objective
We aimed to analyse the mucosal microbiome along the entire gastrointestinal tract within the same individuals.
Methods
Mucosal biopsies were taken from nine different sites in 14 individuals undergoing antegrade and subsequent retrograde double-balloon enteroscopy. The bacterial composition was characterised using 16 S rRNA sequencing with Illumina Miseq.
Results
At double-balloon enteroscopy, one individual had a caecal adenocarcinoma and one individual had Peutz-Jeghers polyps. The composition of the microbiota distinctively changed along the gastrointestinal tract with larger bacterial load, diversity and abundance of
Conclusions
We show that gastrointestinal location is a larger determinant of mucosal microbial diversity than inter-person differences. These data provide a baseline for further studies investigating gastrointestinal microbiota-related disease.
Identifiants
pubmed: 31428414
doi: 10.1177/2050640619852255
pii: 10.1177_2050640619852255
pmc: PMC6683645
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Pagination
897-907Références
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